Advances in the development of a piezoelectric immunosensor for the detection of a tuberculosis biomarker

M. Jaramillo; P. A. Marín; R. A. Torres; E. Pabón; Y. J. Montagut; J. Robledo; M. J. Moreno; J. J. Manclús; A. Montoya

doi:10.1109/IBERSENSOR.2014.6995535

Advances in the development of a piezoelectric immunosensor for the detection of a tuberculosis biomarker

M. Jaramillo, P. A. Marín, R. A. Torres, E. Pabón, Y. J. Montagut, J. Robledo, M. J. Moreno, J. J. Manclús, A. Montoya

Research output: Chapter in Book/Report/Conference proceeding › Conference and proceedings › peer-review

3 Scopus citations

Abstract

The progress in the development of a piezoelectric immunosensor for the detection of a secretion protein (38 kDa) from Mycobacterium tuberculosis are described. A high affinity and specificity monoclonal antibody was obtained from a recombinant form of the target protein. This immunoreagent was employed for the development of a competitive ELISA, as a previous diagnostic test and reference assay for the characterization of the future biosensor. The ELISA detection limit was 14 ng/mL of the 38kDa antigen. The first experimental approach to the piezoelectric immunosensor was undertaken using bovine serum albumin (BSA) as a model antigen and 10 MH piezoelectric crystals as the biosensor transducer element. The obtained results show that high frequency crystals (≥ 100 MHz) should be used in order to reach the high sensitivity required for diagnostic purposes. These crystals would allow obtaining high and stable assay signals using very low protein concentrations for crystal functionalization, thus enhancing immunosensor sensitivity. Preliminary experiments have proved the successful and efficient immobilization of the 38 kDa antigen on high fundamental frequency (HFF) crystals (100 MHz). Moreover, the functionalized surface seems to be able to selectively bind the monoclonal antibody specific to the target antigen.

Original language	English
Title of host publication	2014 IEEE 9th IberoAmerican Congress on Sensors, IBERSENSOR 2014 - Conference Proceedings
Editors	Lorena Garcia
Publisher	Institute of Electrical and Electronics Engineers Inc.
ISBN (Electronic)	9781479968350
DOIs	https://doi.org/10.1109/IBERSENSOR.2014.6995535
State	Published - 22 Dec 2014
Event	2014 IEEE 9th IberoAmerican Congress on Sensors, IBERSENSOR 2014 - Bogota, Colombia Duration: 15 Oct 2014 → 18 Oct 2014

Publication series

Name	2014 IEEE 9th IberoAmerican Congress on Sensors, IBERSENSOR 2014 - Conference Proceedings

Conference

Conference	2014 IEEE 9th IberoAmerican Congress on Sensors, IBERSENSOR 2014
Country/Territory	Colombia
City	Bogota
Period	15/10/14 → 18/10/14

Bibliographical note

Publisher Copyright:
© 2014 IEEE.

Keywords

38 kDa biomarker
BSA
Mycobacterium tuberculosis
monoclonal antibody
piezoelectric immunosensor

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1109/IBERSENSOR.2014.6995535

Cite this

Jaramillo, M., Marín, P. A., Torres, R. A., Pabón, E., Montagut, Y. J., Robledo, J., Moreno, M. J., Manclús, J. J., & Montoya, A. (2014). Advances in the development of a piezoelectric immunosensor for the detection of a tuberculosis biomarker. In L. Garcia (Ed.), 2014 IEEE 9th IberoAmerican Congress on Sensors, IBERSENSOR 2014 - Conference Proceedings Article 6995535 (2014 IEEE 9th IberoAmerican Congress on Sensors, IBERSENSOR 2014 - Conference Proceedings). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/IBERSENSOR.2014.6995535

Jaramillo, M. ; Marín, P. A. ; Torres, R. A. et al. / Advances in the development of a piezoelectric immunosensor for the detection of a tuberculosis biomarker. 2014 IEEE 9th IberoAmerican Congress on Sensors, IBERSENSOR 2014 - Conference Proceedings. editor / Lorena Garcia. Institute of Electrical and Electronics Engineers Inc., 2014. (2014 IEEE 9th IberoAmerican Congress on Sensors, IBERSENSOR 2014 - Conference Proceedings).

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title = "Advances in the development of a piezoelectric immunosensor for the detection of a tuberculosis biomarker",

abstract = "The progress in the development of a piezoelectric immunosensor for the detection of a secretion protein (38 kDa) from Mycobacterium tuberculosis are described. A high affinity and specificity monoclonal antibody was obtained from a recombinant form of the target protein. This immunoreagent was employed for the development of a competitive ELISA, as a previous diagnostic test and reference assay for the characterization of the future biosensor. The ELISA detection limit was 14 ng/mL of the 38kDa antigen. The first experimental approach to the piezoelectric immunosensor was undertaken using bovine serum albumin (BSA) as a model antigen and 10 MH piezoelectric crystals as the biosensor transducer element. The obtained results show that high frequency crystals (≥ 100 MHz) should be used in order to reach the high sensitivity required for diagnostic purposes. These crystals would allow obtaining high and stable assay signals using very low protein concentrations for crystal functionalization, thus enhancing immunosensor sensitivity. Preliminary experiments have proved the successful and efficient immobilization of the 38 kDa antigen on high fundamental frequency (HFF) crystals (100 MHz). Moreover, the functionalized surface seems to be able to selectively bind the monoclonal antibody specific to the target antigen.",

keywords = "38 kDa biomarker, BSA, Mycobacterium tuberculosis, monoclonal antibody, piezoelectric immunosensor",

author = "M. Jaramillo and Mar{\'i}n, {P. A.} and Torres, {R. A.} and E. Pab{\'o}n and Montagut, {Y. J.} and J. Robledo and Moreno, {M. J.} and Mancl{\'u}s, {J. J.} and A. Montoya",

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Jaramillo, M, Marín, PA, Torres, RA, Pabón, E, Montagut, YJ, Robledo, J, Moreno, MJ, Manclús, JJ & Montoya, A 2014, Advances in the development of a piezoelectric immunosensor for the detection of a tuberculosis biomarker. in L Garcia (ed.), 2014 IEEE 9th IberoAmerican Congress on Sensors, IBERSENSOR 2014 - Conference Proceedings., 6995535, 2014 IEEE 9th IberoAmerican Congress on Sensors, IBERSENSOR 2014 - Conference Proceedings, Institute of Electrical and Electronics Engineers Inc., 2014 IEEE 9th IberoAmerican Congress on Sensors, IBERSENSOR 2014, Bogota, Colombia, 15/10/14. https://doi.org/10.1109/IBERSENSOR.2014.6995535

Advances in the development of a piezoelectric immunosensor for the detection of a tuberculosis biomarker. / Jaramillo, M.; Marín, P. A.; Torres, R. A. et al.
2014 IEEE 9th IberoAmerican Congress on Sensors, IBERSENSOR 2014 - Conference Proceedings. ed. / Lorena Garcia. Institute of Electrical and Electronics Engineers Inc., 2014. 6995535 (2014 IEEE 9th IberoAmerican Congress on Sensors, IBERSENSOR 2014 - Conference Proceedings).

Research output: Chapter in Book/Report/Conference proceeding › Conference and proceedings › peer-review

TY - GEN

T1 - Advances in the development of a piezoelectric immunosensor for the detection of a tuberculosis biomarker

AU - Jaramillo, M.

AU - Marín, P. A.

AU - Torres, R. A.

AU - Pabón, E.

AU - Montagut, Y. J.

AU - Robledo, J.

AU - Moreno, M. J.

AU - Manclús, J. J.

AU - Montoya, A.

PY - 2014/12/22

Y1 - 2014/12/22

N2 - The progress in the development of a piezoelectric immunosensor for the detection of a secretion protein (38 kDa) from Mycobacterium tuberculosis are described. A high affinity and specificity monoclonal antibody was obtained from a recombinant form of the target protein. This immunoreagent was employed for the development of a competitive ELISA, as a previous diagnostic test and reference assay for the characterization of the future biosensor. The ELISA detection limit was 14 ng/mL of the 38kDa antigen. The first experimental approach to the piezoelectric immunosensor was undertaken using bovine serum albumin (BSA) as a model antigen and 10 MH piezoelectric crystals as the biosensor transducer element. The obtained results show that high frequency crystals (≥ 100 MHz) should be used in order to reach the high sensitivity required for diagnostic purposes. These crystals would allow obtaining high and stable assay signals using very low protein concentrations for crystal functionalization, thus enhancing immunosensor sensitivity. Preliminary experiments have proved the successful and efficient immobilization of the 38 kDa antigen on high fundamental frequency (HFF) crystals (100 MHz). Moreover, the functionalized surface seems to be able to selectively bind the monoclonal antibody specific to the target antigen.

AB - The progress in the development of a piezoelectric immunosensor for the detection of a secretion protein (38 kDa) from Mycobacterium tuberculosis are described. A high affinity and specificity monoclonal antibody was obtained from a recombinant form of the target protein. This immunoreagent was employed for the development of a competitive ELISA, as a previous diagnostic test and reference assay for the characterization of the future biosensor. The ELISA detection limit was 14 ng/mL of the 38kDa antigen. The first experimental approach to the piezoelectric immunosensor was undertaken using bovine serum albumin (BSA) as a model antigen and 10 MH piezoelectric crystals as the biosensor transducer element. The obtained results show that high frequency crystals (≥ 100 MHz) should be used in order to reach the high sensitivity required for diagnostic purposes. These crystals would allow obtaining high and stable assay signals using very low protein concentrations for crystal functionalization, thus enhancing immunosensor sensitivity. Preliminary experiments have proved the successful and efficient immobilization of the 38 kDa antigen on high fundamental frequency (HFF) crystals (100 MHz). Moreover, the functionalized surface seems to be able to selectively bind the monoclonal antibody specific to the target antigen.

KW - 38 kDa biomarker

KW - BSA

KW - Mycobacterium tuberculosis

KW - monoclonal antibody

KW - piezoelectric immunosensor

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U2 - 10.1109/IBERSENSOR.2014.6995535

DO - 10.1109/IBERSENSOR.2014.6995535

M3 - Ponencia publicada en las memorias del evento con ISBN

AN - SCOPUS:84921690296

T3 - 2014 IEEE 9th IberoAmerican Congress on Sensors, IBERSENSOR 2014 - Conference Proceedings

BT - 2014 IEEE 9th IberoAmerican Congress on Sensors, IBERSENSOR 2014 - Conference Proceedings

A2 - Garcia, Lorena

PB - Institute of Electrical and Electronics Engineers Inc.

T2 - 2014 IEEE 9th IberoAmerican Congress on Sensors, IBERSENSOR 2014

Y2 - 15 October 2014 through 18 October 2014

ER -

Jaramillo M, Marín PA, Torres RA, Pabón E, Montagut YJ, Robledo J et al. Advances in the development of a piezoelectric immunosensor for the detection of a tuberculosis biomarker. In Garcia L, editor, 2014 IEEE 9th IberoAmerican Congress on Sensors, IBERSENSOR 2014 - Conference Proceedings. Institute of Electrical and Electronics Engineers Inc. 2014. 6995535. (2014 IEEE 9th IberoAmerican Congress on Sensors, IBERSENSOR 2014 - Conference Proceedings). doi: 10.1109/IBERSENSOR.2014.6995535

Advances in the development of a piezoelectric immunosensor for the detection of a tuberculosis biomarker

Abstract

Publication series

Conference

Bibliographical note

Keywords

UN SDGs

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