Assessment of mycobacteremia detection as a complementary method for the diagnosis of tuberculosis in HIV-infected patients

J. Hernández, A. Jaramillo, G. I. Mejía, P. Barón, V. Gomez, M. A. Restrepo, J. Robledo

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    6 Scopus citations


    The purpose of this investigation was to assess the usefulness of mycobacteremia detection in human immunodeficiency virus (HIV) patients with suspected tuberculosis. The study included 47 patients with suspected tuberculosis and confirmed HIV infection. A first blood sample was incubated in a BACTEC 9050 MB system, while white blood cells isolation was performed on a second blood specimen before incubation in a BACTEC MGIT 960 system. The third specimen was taken from the affected organs of each patient according to their clinical profile. Twelve (25.5%) patients were positive for mycobacterial infection identified by any of the methods used. Ten (21.2%) were positive for Mycobacterium tuberculosis and 2 (4.3%) for M. avium. Six patients were diagnosed by the culture of specimen from affected organs only, whilst three other patients were positive exclusively for blood cultures. Three additional patients were diagnosed by both methods. Four patients with negative cultures were ultimately diagnosed with tuberculosis by measuring the adenosine deaminase levels. Mycobacteremia detection can be used to increase the sensitivity of the diagnosis of tuberculosis and other mycobacteria in patients with HIV. However, it cannot be used as the sole diagnostic method. Clinical specimen cultures do not provide 100% diagnostic accuracy and it is, therefore, critical to further improve the mycobacteria detection sensitivity.

    Original languageEnglish
    Pages (from-to)1435-1441
    Number of pages7
    JournalEuropean Journal of Clinical Microbiology and Infectious Diseases
    Issue number11
    StatePublished - Nov 2010

    Bibliographical note

    Funding Information:
    Acknowledgments The authors wish to thank Natalia Builes, Angela Guzman, and Elsa Zapata in the startup process of the present work, as well as Nidia Correa, Carlos A. Agudelo, and Angela M. Tobon who assessed this project. Also, we wish to acknowledge the support of the medical staff from Hospital La Maria Dinamica Clinical Laboratory, Hospital Pablo Tobon Uribe, Clinica Universitaria Bolivariana, and Universidad Pontificia Bolivariana. This work was financed by CIDI (797-11/05-44) from the Universidad Pontificia Bolivariana and the Corporación para Investigaciones Biológicas (CIB).


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