Bacterial cellulose produced by a new acid-resistant strain of Gluconacetobacter genus

Cristina Castro; Robin Zuluaga; Catalina Álvarez; Jean Luc Putaux; Gloria Caro; Orlando J. Rojas; Iñaki Mondragon; Piedad Gañán

doi:10.1016/j.carbpol.2012.03.045

Bacterial cellulose produced by a new acid-resistant strain of Gluconacetobacter genus

Cristina Castro
, Robin Zuluaga
, Catalina Álvarez
, Jean Luc Putaux
, Gloria Caro
, Orlando J. Rojas
, Iñaki Mondragon
, Piedad Gañán

Research output: Contribution to scientific journal › Article in an indexed scientific journal › peer-review

220 Scopus citations

Abstract

A bacterial strain isolated from the fermentation of Colombian homemade vinegar, Gluconacetobacter medellensis, was investigated as a new source of bacterial cellulose (BC). The BC produced from substrate media consisting of various carbon sources at different pH and incubation times was quantified. Hestrin-Schramm (HS) medium modified with glucose led to the highest BC yields followed by sucrose and fructose. Interestingly, the microorganisms are highly tolerant to low pH: an optimum yield of 4.5 g/L was achieved at pH 3.5, which is generally too low for other bacterial species to function. The cellulose microfibrils produced by the new strain were characterized by scanning and transmission electron microscopy, infrared spectroscopy X-ray diffraction and elemental analysis. The morphological, structural and chemical characteristics of the cellulose produced are similar to those expected for BC.

Original language	English
Pages (from-to)	1033-1037
Number of pages	5
Journal	Carbohydrate Polymers
Volume	89
Issue number	4
DOIs	https://doi.org/10.1016/j.carbpol.2012.03.045
State	Published - 1 Aug 2012

Bibliographical note

Funding Information:
The authors acknowledge Colombia's Colciencias and SENA for financial support as well as the microbiology laboratory of the School of Health Sciences at the Universidad Pontificia Bolivariana (Medellín-Colombia) by the technical support for the preliminary microbiological analysis.

Keywords

Acid resistance bacteria
Bacterial cellulose
Cellulose production
Gluconacetobacter medellensis
Microfibril

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10.1016/j.carbpol.2012.03.045

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title = "Bacterial cellulose produced by a new acid-resistant strain of Gluconacetobacter genus",

abstract = "A bacterial strain isolated from the fermentation of Colombian homemade vinegar, Gluconacetobacter medellensis, was investigated as a new source of bacterial cellulose (BC). The BC produced from substrate media consisting of various carbon sources at different pH and incubation times was quantified. Hestrin-Schramm (HS) medium modified with glucose led to the highest BC yields followed by sucrose and fructose. Interestingly, the microorganisms are highly tolerant to low pH: an optimum yield of 4.5 g/L was achieved at pH 3.5, which is generally too low for other bacterial species to function. The cellulose microfibrils produced by the new strain were characterized by scanning and transmission electron microscopy, infrared spectroscopy X-ray diffraction and elemental analysis. The morphological, structural and chemical characteristics of the cellulose produced are similar to those expected for BC.",

keywords = "Acid resistance bacteria, Bacterial cellulose, Cellulose production, Gluconacetobacter medellensis, Microfibril",

author = "Cristina Castro and Robin Zuluaga and Catalina {\'A}lvarez and Putaux, \{Jean Luc\} and Gloria Caro and Rojas, \{Orlando J.\} and I{\~n}aki Mondragon and Piedad Ga{\~n}{\'a}n",

note = "Funding Information: The authors acknowledge Colombia's Colciencias and SENA for financial support as well as the microbiology laboratory of the School of Health Sciences at the Universidad Pontificia Bolivariana (Medell{\'i}n-Colombia) by the technical support for the preliminary microbiological analysis.",

year = "2012",

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doi = "10.1016/j.carbpol.2012.03.045",

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AU - Castro, Cristina

AU - Zuluaga, Robin

AU - Álvarez, Catalina

AU - Putaux, Jean Luc

AU - Caro, Gloria

AU - Rojas, Orlando J.

AU - Mondragon, Iñaki

AU - Gañán, Piedad

N1 - Funding Information: The authors acknowledge Colombia's Colciencias and SENA for financial support as well as the microbiology laboratory of the School of Health Sciences at the Universidad Pontificia Bolivariana (Medellín-Colombia) by the technical support for the preliminary microbiological analysis.

PY - 2012/8/1

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N2 - A bacterial strain isolated from the fermentation of Colombian homemade vinegar, Gluconacetobacter medellensis, was investigated as a new source of bacterial cellulose (BC). The BC produced from substrate media consisting of various carbon sources at different pH and incubation times was quantified. Hestrin-Schramm (HS) medium modified with glucose led to the highest BC yields followed by sucrose and fructose. Interestingly, the microorganisms are highly tolerant to low pH: an optimum yield of 4.5 g/L was achieved at pH 3.5, which is generally too low for other bacterial species to function. The cellulose microfibrils produced by the new strain were characterized by scanning and transmission electron microscopy, infrared spectroscopy X-ray diffraction and elemental analysis. The morphological, structural and chemical characteristics of the cellulose produced are similar to those expected for BC.

AB - A bacterial strain isolated from the fermentation of Colombian homemade vinegar, Gluconacetobacter medellensis, was investigated as a new source of bacterial cellulose (BC). The BC produced from substrate media consisting of various carbon sources at different pH and incubation times was quantified. Hestrin-Schramm (HS) medium modified with glucose led to the highest BC yields followed by sucrose and fructose. Interestingly, the microorganisms are highly tolerant to low pH: an optimum yield of 4.5 g/L was achieved at pH 3.5, which is generally too low for other bacterial species to function. The cellulose microfibrils produced by the new strain were characterized by scanning and transmission electron microscopy, infrared spectroscopy X-ray diffraction and elemental analysis. The morphological, structural and chemical characteristics of the cellulose produced are similar to those expected for BC.

KW - Acid resistance bacteria

KW - Bacterial cellulose

KW - Cellulose production

KW - Gluconacetobacter medellensis

KW - Microfibril

UR - https://www.scopus.com/pages/publications/84861623001

U2 - 10.1016/j.carbpol.2012.03.045

DO - 10.1016/j.carbpol.2012.03.045

M3 - Artículo en revista científica indexada

C2 - 24750910

AN - SCOPUS:84861623001

SN - 0144-8617

VL - 89

SP - 1033

EP - 1037

JO - Carbohydrate Polymers

JF - Carbohydrate Polymers

IS - 4

ER -

Bacterial cellulose produced by a new acid-resistant strain of Gluconacetobacter genus

Abstract

Bibliographical note

Keywords

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