Characterization of Mycobacterium tuberculosis var. africanum isolated from a patient with pulmonary tuberculosis in Brazil

Marcelo Fouad Rabahi; Emilyn Costa Conceição; Luísa Oliveira de Paiva; Marcos Vinícius Muniz Lemes Souto; Maria Carolina Sisco; Jacobus de Waard; Paulo Cesar de Souza Caldas; Fatima Fandinho; Jesus Pais Ramos; Luciana Distasio de Carvalho; Carlos Eduardo Dias Campos; Karla Valéria Batista Lima; Sandro Patroca da Silva; Abhinav Sharma; Jaime Robledo; Uriel Alonso Hurtado Paez; Rafael Silva Duarte; Marlei Gomes da Silva; Lia Lima Gomes; Sidra Ezidio Gonçalves Vasconcellos; Cecile Uwezeye; Bouke C. de Jong; Ana Paula Junqueira-Kipnis; Philip Noel Suffys

doi:10.1016/j.meegid.2020.104550

Characterization of Mycobacterium tuberculosis var. africanum isolated from a patient with pulmonary tuberculosis in Brazil

Marcelo Fouad Rabahi, Emilyn Costa Conceição, Luísa Oliveira de Paiva, Marcos Vinícius Muniz Lemes Souto, Maria Carolina Sisco, Jacobus de Waard, Paulo Cesar de Souza Caldas, Fatima Fandinho, Jesus Pais Ramos, Luciana Distasio de Carvalho, Carlos Eduardo Dias Campos, Karla Valéria Batista Lima, Sandro Patroca da Silva, Abhinav Sharma, Jaime Robledo, Uriel Alonso Hurtado Paez, Rafael Silva Duarte, Marlei Gomes da Silva, Lia Lima Gomes, Sidra Ezidio Gonçalves VasconcellosCecile Uwezeye, Bouke C. de Jong, Ana Paula Junqueira-Kipnis, Philip Noel Suffys

Research output: Contribution to journal › Article in an indexed scientific journal › peer-review

5 Scopus citations

Abstract

Human tuberculosis (TB) is caused by members of the Mycobacterium tuberculosis complex (MTBC), including Mycobacterium tuberculosis var. tuberculosis (MTB) and Mycobacterium tuberculosis var. africanum (MAF). While MTB is isolated worldwide, MAF is almost completely restricted to the African continent, and despite the historical proximity between Brazil and Africa during the slave trade, no case of TB being caused by MAF has been reported in Brazil to date. We hereby describe the first case of TB caused by MAF in Brazil comparing its genome against the published ones. A female patient who had never visited Africa presented with clinical symptoms typical of pulmonary TB. Based on 16S rRNA gene sequencing, the cultured isolate was identified as belonging to MTBC and partial sequence of the hsp65 gene was identical to that of MAF. This was confirmed by genotyping based on detection of Single Nucleotide Polymorphism (SNP), Region of Difference (RD) and spoligotyping. The isolate presented the Shared International Typing (SIT) 181. In the whole-genome comparison against MAF genomes available on published EMBL-EBI European Nucleotide Archive (ENA), the Brazilian genome (MAFBRA00707) was identified as belonging to Lineage 6 and clustered with isolates from The Gambia. This is the first report of the isolation of MAF from a patient from Brazil, without evidence of having any contact with an African index case.

Original language	English
Article number	104550
Journal	Infection, Genetics and Evolution
Volume	85
DOIs	https://doi.org/10.1016/j.meegid.2020.104550
State	Published - Nov 2020
Externally published	Yes

Bibliographical note

Publisher Copyright:
© 2020 Elsevier B.V.

Keywords

Brazil
Case report
Genome
Molecular epidemiology
Mycobacterium tuberculosis var. africanum
Tuberculosis

Types Minciencias

Artículos de investigación con calidad A1 / Q1

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/j.meegid.2020.104550

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Rabahi, M. F., Conceição, E. C., de Paiva, L. O., Souto, M. V. M. L., Sisco, M. C., de Waard, J., de Souza Caldas, P. C., Fandinho, F., Ramos, J. P., de Carvalho, L. D., Campos, C. E. D., Lima, K. V. B., da Silva, S. P., Sharma, A., Robledo, J., Paez, U. A. H., Duarte, R. S., da Silva, M. G., Gomes, L. L., ... Suffys, P. N. (2020). Characterization of Mycobacterium tuberculosis var. africanum isolated from a patient with pulmonary tuberculosis in Brazil. Infection, Genetics and Evolution, 85, Article 104550. https://doi.org/10.1016/j.meegid.2020.104550

@article{81126e034fe949e78a2a68fb5de8dbb6,

title = "Characterization of Mycobacterium tuberculosis var. africanum isolated from a patient with pulmonary tuberculosis in Brazil",

abstract = "Human tuberculosis (TB) is caused by members of the Mycobacterium tuberculosis complex (MTBC), including Mycobacterium tuberculosis var. tuberculosis (MTB) and Mycobacterium tuberculosis var. africanum (MAF). While MTB is isolated worldwide, MAF is almost completely restricted to the African continent, and despite the historical proximity between Brazil and Africa during the slave trade, no case of TB being caused by MAF has been reported in Brazil to date. We hereby describe the first case of TB caused by MAF in Brazil comparing its genome against the published ones. A female patient who had never visited Africa presented with clinical symptoms typical of pulmonary TB. Based on 16S rRNA gene sequencing, the cultured isolate was identified as belonging to MTBC and partial sequence of the hsp65 gene was identical to that of MAF. This was confirmed by genotyping based on detection of Single Nucleotide Polymorphism (SNP), Region of Difference (RD) and spoligotyping. The isolate presented the Shared International Typing (SIT) 181. In the whole-genome comparison against MAF genomes available on published EMBL-EBI European Nucleotide Archive (ENA), the Brazilian genome (MAFBRA00707) was identified as belonging to Lineage 6 and clustered with isolates from The Gambia. This is the first report of the isolation of MAF from a patient from Brazil, without evidence of having any contact with an African index case.",

keywords = "Brazil, Case report, Genome, Molecular epidemiology, Mycobacterium tuberculosis var. africanum, Tuberculosis",

author = "Rabahi, {Marcelo Fouad} and Concei{\c c}{\~a}o, {Emilyn Costa} and {de Paiva}, {Lu{\'i}sa Oliveira} and Souto, {Marcos Vin{\'i}cius Muniz Lemes} and Sisco, {Maria Carolina} and {de Waard}, Jacobus and {de Souza Caldas}, {Paulo Cesar} and Fatima Fandinho and Ramos, {Jesus Pais} and {de Carvalho}, {Luciana Distasio} and Campos, {Carlos Eduardo Dias} and Lima, {Karla Val{\'e}ria Batista} and {da Silva}, {Sandro Patroca} and Abhinav Sharma and Jaime Robledo and Paez, {Uriel Alonso Hurtado} and Duarte, {Rafael Silva} and {da Silva}, {Marlei Gomes} and Gomes, {Lia Lima} and Vasconcellos, {Sidra Ezidio Gon{\c c}alves} and Cecile Uwezeye and {de Jong}, {Bouke C.} and Junqueira-Kipnis, {Ana Paula} and Suffys, {Philip Noel}",

note = "Publisher Copyright: {\textcopyright} 2020 Elsevier B.V.",

year = "2020",

month = nov,

doi = "10.1016/j.meegid.2020.104550",

language = "Ingl{\'e}s",

volume = "85",

journal = "Infection, Genetics and Evolution",

issn = "1567-1348",

publisher = "Elsevier B.V.",

}

Rabahi, MF, Conceição, EC, de Paiva, LO, Souto, MVML, Sisco, MC, de Waard, J, de Souza Caldas, PC, Fandinho, F, Ramos, JP, de Carvalho, LD, Campos, CED, Lima, KVB, da Silva, SP, Sharma, A, Robledo, J, Paez, UAH, Duarte, RS, da Silva, MG, Gomes, LL, Vasconcellos, SEG, Uwezeye, C, de Jong, BC, Junqueira-Kipnis, AP & Suffys, PN 2020, 'Characterization of Mycobacterium tuberculosis var. africanum isolated from a patient with pulmonary tuberculosis in Brazil', Infection, Genetics and Evolution, vol. 85, 104550. https://doi.org/10.1016/j.meegid.2020.104550

Characterization of Mycobacterium tuberculosis var. africanum isolated from a patient with pulmonary tuberculosis in Brazil. / Rabahi, Marcelo Fouad; Conceição, Emilyn Costa; de Paiva, Luísa Oliveira et al.
In: Infection, Genetics and Evolution, Vol. 85, 104550, 11.2020.

Research output: Contribution to journal › Article in an indexed scientific journal › peer-review

TY - JOUR

T1 - Characterization of Mycobacterium tuberculosis var. africanum isolated from a patient with pulmonary tuberculosis in Brazil

AU - Rabahi, Marcelo Fouad

AU - Conceição, Emilyn Costa

AU - de Paiva, Luísa Oliveira

AU - Souto, Marcos Vinícius Muniz Lemes

AU - Sisco, Maria Carolina

AU - de Waard, Jacobus

AU - de Souza Caldas, Paulo Cesar

AU - Fandinho, Fatima

AU - Ramos, Jesus Pais

AU - de Carvalho, Luciana Distasio

AU - Campos, Carlos Eduardo Dias

AU - Lima, Karla Valéria Batista

AU - da Silva, Sandro Patroca

AU - Sharma, Abhinav

AU - Robledo, Jaime

AU - Paez, Uriel Alonso Hurtado

AU - Duarte, Rafael Silva

AU - da Silva, Marlei Gomes

AU - Gomes, Lia Lima

AU - Vasconcellos, Sidra Ezidio Gonçalves

AU - Uwezeye, Cecile

AU - de Jong, Bouke C.

AU - Junqueira-Kipnis, Ana Paula

AU - Suffys, Philip Noel

PY - 2020/11

Y1 - 2020/11

N2 - Human tuberculosis (TB) is caused by members of the Mycobacterium tuberculosis complex (MTBC), including Mycobacterium tuberculosis var. tuberculosis (MTB) and Mycobacterium tuberculosis var. africanum (MAF). While MTB is isolated worldwide, MAF is almost completely restricted to the African continent, and despite the historical proximity between Brazil and Africa during the slave trade, no case of TB being caused by MAF has been reported in Brazil to date. We hereby describe the first case of TB caused by MAF in Brazil comparing its genome against the published ones. A female patient who had never visited Africa presented with clinical symptoms typical of pulmonary TB. Based on 16S rRNA gene sequencing, the cultured isolate was identified as belonging to MTBC and partial sequence of the hsp65 gene was identical to that of MAF. This was confirmed by genotyping based on detection of Single Nucleotide Polymorphism (SNP), Region of Difference (RD) and spoligotyping. The isolate presented the Shared International Typing (SIT) 181. In the whole-genome comparison against MAF genomes available on published EMBL-EBI European Nucleotide Archive (ENA), the Brazilian genome (MAFBRA00707) was identified as belonging to Lineage 6 and clustered with isolates from The Gambia. This is the first report of the isolation of MAF from a patient from Brazil, without evidence of having any contact with an African index case.

AB - Human tuberculosis (TB) is caused by members of the Mycobacterium tuberculosis complex (MTBC), including Mycobacterium tuberculosis var. tuberculosis (MTB) and Mycobacterium tuberculosis var. africanum (MAF). While MTB is isolated worldwide, MAF is almost completely restricted to the African continent, and despite the historical proximity between Brazil and Africa during the slave trade, no case of TB being caused by MAF has been reported in Brazil to date. We hereby describe the first case of TB caused by MAF in Brazil comparing its genome against the published ones. A female patient who had never visited Africa presented with clinical symptoms typical of pulmonary TB. Based on 16S rRNA gene sequencing, the cultured isolate was identified as belonging to MTBC and partial sequence of the hsp65 gene was identical to that of MAF. This was confirmed by genotyping based on detection of Single Nucleotide Polymorphism (SNP), Region of Difference (RD) and spoligotyping. The isolate presented the Shared International Typing (SIT) 181. In the whole-genome comparison against MAF genomes available on published EMBL-EBI European Nucleotide Archive (ENA), the Brazilian genome (MAFBRA00707) was identified as belonging to Lineage 6 and clustered with isolates from The Gambia. This is the first report of the isolation of MAF from a patient from Brazil, without evidence of having any contact with an African index case.

KW - Brazil

KW - Case report

KW - Genome

KW - Molecular epidemiology

KW - Mycobacterium tuberculosis var. africanum

KW - Tuberculosis

UR - http://www.scopus.com/inward/record.url?scp=85091210560&partnerID=8YFLogxK

U2 - 10.1016/j.meegid.2020.104550

DO - 10.1016/j.meegid.2020.104550

M3 - Artículo en revista científica indexada

C2 - 32920193

AN - SCOPUS:85091210560

SN - 1567-1348

VL - 85

JO - Infection, Genetics and Evolution

JF - Infection, Genetics and Evolution

M1 - 104550

ER -

Characterization of Mycobacterium tuberculosis var. africanum isolated from a patient with pulmonary tuberculosis in Brazil

Abstract

Bibliographical note

Keywords

Types Minciencias

UN SDGs

Access to Document

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Cite this