Diffuse Reflectance Parameters of Treated Leishmaniasis Cutaneous Ulcers and Association with Histopathologies in an Animal Model: A Proof of Concept

Lina Restrepo; Javier Murillo; Deivid Botina; Artur Zarzycki; Johnson Garzón; Ricardo Franco; Jaime Montano; Samuel Calderon; Maria C. Torres-Madronero; Franck Marzani; Sara M. Robledo; July Galeano

doi:10.1177/24726303211030292

Diffuse Reflectance Parameters of Treated Leishmaniasis Cutaneous Ulcers and Association with Histopathologies in an Animal Model: A Proof of Concept

Lina Restrepo, Javier Murillo, Deivid Botina, Artur Zarzycki, Johnson Garzón, Ricardo Franco, Jaime Montano, Samuel Calderon, Maria C. Torres-Madronero, Franck Marzani, Sara M. Robledo, July Galeano

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Cutaneous leishmaniasis (CL) is a parasitic disease that produces chronic skin ulcers. Although it has a worldwide presence, it is a neglected disease that still requires novel tools for its management. In order to study the use of optical tools in CL, this article presents a preliminary study of the correlation between CL histopathological and optical parameters. Optical parameters correspond to absorption and scattering coefficients obtained from diffuse reflectance spectra of treated CL in golden hamsters. Independently, histopathological data were collected from the same hamsters. As a result, after Spearman correlation and the Kruskal–Wallis test, inverse correlation was found between absorption/scattering optical parameters and inflammatory histopathological values, such as the scattering parameter related to the diameter of fibroblasts with the histopathological parameters of fibrosis, polymorphonuclear neutrophils, lymphocytes, plasmocytes, hyperplasia, and Leishmania, and the absorption parameter oxygen saturation showed a relation with the granulation tissue histopathological parameter. These correlations agree with the expected behavior of tissue composition during the healing process in CL. The results correspond to a proof of concept that shows that optical diffuse reflectance-based tools and methods could be considered as an alternative to assist in CL diagnosis and treatment follow-up.

Original language	English
Pages (from-to)	667-680
Number of pages	14
Journal	SLAS Technology
Volume	26
Issue number	6
DOIs	https://doi.org/10.1177/24726303211030292
State	Published - Dec 2021

Bibliographical note

Funding Information:
The authors disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: The authors would like to acknowledge the financial support given by MINCIENCIAS (Colombia), Instituto Tecnológico Metropolitano, Universidad de Antioquia, Universidad Pontificia Bolivariana, and Kinetics Systems S.A.S. (Medellín-Colombia), under project number 115077757186.

Funding Information:
The authors would like to acknowledge the support given by Dr. Carlos Muskus from PECET Laboratory in Universidad de Antioquia (UdeA), the histopathological laboratory of UdeA, the PECET bioterium Staff (Medellín-Colombia), and Dr. Jorge Arevalo from Universidad Peruana Cayetano Heredia (Lima-Perú). The authors disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: The authors would like to acknowledge the financial support given by MINCIENCIAS (Colombia), Instituto Tecnológico Metropolitano, Universidad de Antioquia, Universidad Pontificia Bolivariana, and Kinetics Systems S.A.S. (Medellín-Colombia), under project number 115077757186.

Publisher Copyright:
© Society for Laboratory Automation and Screening 2021.

Keywords

animal model
cutaneous leishmaniasis
cutaneous ulcers
diffuse reflectance
histopathologies

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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Restrepo, L., Murillo, J., Botina, D., Zarzycki, A., Garzón, J., Franco, R., Montano, J., Calderon, S., Torres-Madronero, M. C., Marzani, F., Robledo, S. M., & Galeano, J. (2021). Diffuse Reflectance Parameters of Treated Leishmaniasis Cutaneous Ulcers and Association with Histopathologies in an Animal Model: A Proof of Concept. SLAS Technology, 26(6), 667-680. https://doi.org/10.1177/24726303211030292

Restrepo, Lina ; Murillo, Javier ; Botina, Deivid ; Zarzycki, Artur ; Garzón, Johnson ; Franco, Ricardo ; Montano, Jaime ; Calderon, Samuel ; Torres-Madronero, Maria C. ; Marzani, Franck ; Robledo, Sara M. ; Galeano, July. / Diffuse Reflectance Parameters of Treated Leishmaniasis Cutaneous Ulcers and Association with Histopathologies in an Animal Model : A Proof of Concept. In: SLAS Technology. 2021 ; Vol. 26, No. 6. pp. 667-680.

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title = "Diffuse Reflectance Parameters of Treated Leishmaniasis Cutaneous Ulcers and Association with Histopathologies in an Animal Model: A Proof of Concept",

abstract = "Cutaneous leishmaniasis (CL) is a parasitic disease that produces chronic skin ulcers. Although it has a worldwide presence, it is a neglected disease that still requires novel tools for its management. In order to study the use of optical tools in CL, this article presents a preliminary study of the correlation between CL histopathological and optical parameters. Optical parameters correspond to absorption and scattering coefficients obtained from diffuse reflectance spectra of treated CL in golden hamsters. Independently, histopathological data were collected from the same hamsters. As a result, after Spearman correlation and the Kruskal–Wallis test, inverse correlation was found between absorption/scattering optical parameters and inflammatory histopathological values, such as the scattering parameter related to the diameter of fibroblasts with the histopathological parameters of fibrosis, polymorphonuclear neutrophils, lymphocytes, plasmocytes, hyperplasia, and Leishmania, and the absorption parameter oxygen saturation showed a relation with the granulation tissue histopathological parameter. These correlations agree with the expected behavior of tissue composition during the healing process in CL. The results correspond to a proof of concept that shows that optical diffuse reflectance-based tools and methods could be considered as an alternative to assist in CL diagnosis and treatment follow-up.",

keywords = "animal model, cutaneous leishmaniasis, cutaneous ulcers, diffuse reflectance, histopathologies",

author = "Lina Restrepo and Javier Murillo and Deivid Botina and Artur Zarzycki and Johnson Garz{\'o}n and Ricardo Franco and Jaime Montano and Samuel Calderon and Torres-Madronero, {Maria C.} and Franck Marzani and Robledo, {Sara M.} and July Galeano",

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year = "2021",

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doi = "10.1177/24726303211030292",

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Restrepo, L, Murillo, J, Botina, D, Zarzycki, A, Garzón, J, Franco, R, Montano, J, Calderon, S, Torres-Madronero, MC, Marzani, F, Robledo, SM & Galeano, J 2021, 'Diffuse Reflectance Parameters of Treated Leishmaniasis Cutaneous Ulcers and Association with Histopathologies in an Animal Model: A Proof of Concept', SLAS Technology, vol. 26, no. 6, pp. 667-680. https://doi.org/10.1177/24726303211030292

Diffuse Reflectance Parameters of Treated Leishmaniasis Cutaneous Ulcers and Association with Histopathologies in an Animal Model : A Proof of Concept. / Restrepo, Lina; Murillo, Javier; Botina, Deivid; Zarzycki, Artur; Garzón, Johnson; Franco, Ricardo; Montano, Jaime; Calderon, Samuel; Torres-Madronero, Maria C.; Marzani, Franck; Robledo, Sara M.; Galeano, July.

In: SLAS Technology, Vol. 26, No. 6, 12.2021, p. 667-680.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Diffuse Reflectance Parameters of Treated Leishmaniasis Cutaneous Ulcers and Association with Histopathologies in an Animal Model

T2 - A Proof of Concept

AU - Restrepo, Lina

AU - Murillo, Javier

AU - Botina, Deivid

AU - Zarzycki, Artur

AU - Garzón, Johnson

AU - Franco, Ricardo

AU - Montano, Jaime

AU - Calderon, Samuel

AU - Torres-Madronero, Maria C.

AU - Marzani, Franck

AU - Robledo, Sara M.

AU - Galeano, July

PY - 2021/12

Y1 - 2021/12

N2 - Cutaneous leishmaniasis (CL) is a parasitic disease that produces chronic skin ulcers. Although it has a worldwide presence, it is a neglected disease that still requires novel tools for its management. In order to study the use of optical tools in CL, this article presents a preliminary study of the correlation between CL histopathological and optical parameters. Optical parameters correspond to absorption and scattering coefficients obtained from diffuse reflectance spectra of treated CL in golden hamsters. Independently, histopathological data were collected from the same hamsters. As a result, after Spearman correlation and the Kruskal–Wallis test, inverse correlation was found between absorption/scattering optical parameters and inflammatory histopathological values, such as the scattering parameter related to the diameter of fibroblasts with the histopathological parameters of fibrosis, polymorphonuclear neutrophils, lymphocytes, plasmocytes, hyperplasia, and Leishmania, and the absorption parameter oxygen saturation showed a relation with the granulation tissue histopathological parameter. These correlations agree with the expected behavior of tissue composition during the healing process in CL. The results correspond to a proof of concept that shows that optical diffuse reflectance-based tools and methods could be considered as an alternative to assist in CL diagnosis and treatment follow-up.

AB - Cutaneous leishmaniasis (CL) is a parasitic disease that produces chronic skin ulcers. Although it has a worldwide presence, it is a neglected disease that still requires novel tools for its management. In order to study the use of optical tools in CL, this article presents a preliminary study of the correlation between CL histopathological and optical parameters. Optical parameters correspond to absorption and scattering coefficients obtained from diffuse reflectance spectra of treated CL in golden hamsters. Independently, histopathological data were collected from the same hamsters. As a result, after Spearman correlation and the Kruskal–Wallis test, inverse correlation was found between absorption/scattering optical parameters and inflammatory histopathological values, such as the scattering parameter related to the diameter of fibroblasts with the histopathological parameters of fibrosis, polymorphonuclear neutrophils, lymphocytes, plasmocytes, hyperplasia, and Leishmania, and the absorption parameter oxygen saturation showed a relation with the granulation tissue histopathological parameter. These correlations agree with the expected behavior of tissue composition during the healing process in CL. The results correspond to a proof of concept that shows that optical diffuse reflectance-based tools and methods could be considered as an alternative to assist in CL diagnosis and treatment follow-up.

KW - animal model

KW - cutaneous leishmaniasis

KW - cutaneous ulcers

KW - diffuse reflectance

KW - histopathologies

UR - http://www.scopus.com/inward/record.url?scp=85111343453&partnerID=8YFLogxK

U2 - 10.1177/24726303211030292

DO - 10.1177/24726303211030292

M3 - Artículo

AN - SCOPUS:85111343453

VL - 26

SP - 667

EP - 680

JO - SLAS Technology

JF - SLAS Technology

SN - 2472-6303

IS - 6

ER -

Diffuse Reflectance Parameters of Treated Leishmaniasis Cutaneous Ulcers and Association with Histopathologies in an Animal Model: A Proof of Concept

Abstract

Bibliographical note

Keywords

UN SDGs

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