Diffuse Reflectance Parameters of Treated Leishmaniasis Cutaneous Ulcers and Association with Histopathologies in an Animal Model: A Proof of Concept

Lina Restrepo, Javier Murillo, Deivid Botina, Artur Zarzycki, Johnson Garzón, Ricardo Franco, Jaime Montano, Samuel Calderon, Maria C. Torres-Madronero, Franck Marzani, Sara M. Robledo, July Galeano

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    1 Scopus citations

    Abstract

    Cutaneous leishmaniasis (CL) is a parasitic disease that produces chronic skin ulcers. Although it has a worldwide presence, it is a neglected disease that still requires novel tools for its management. In order to study the use of optical tools in CL, this article presents a preliminary study of the correlation between CL histopathological and optical parameters. Optical parameters correspond to absorption and scattering coefficients obtained from diffuse reflectance spectra of treated CL in golden hamsters. Independently, histopathological data were collected from the same hamsters. As a result, after Spearman correlation and the Kruskal–Wallis test, inverse correlation was found between absorption/scattering optical parameters and inflammatory histopathological values, such as the scattering parameter related to the diameter of fibroblasts with the histopathological parameters of fibrosis, polymorphonuclear neutrophils, lymphocytes, plasmocytes, hyperplasia, and Leishmania, and the absorption parameter oxygen saturation showed a relation with the granulation tissue histopathological parameter. These correlations agree with the expected behavior of tissue composition during the healing process in CL. The results correspond to a proof of concept that shows that optical diffuse reflectance-based tools and methods could be considered as an alternative to assist in CL diagnosis and treatment follow-up.

    Original languageEnglish
    Pages (from-to)667-680
    Number of pages14
    JournalSLAS Technology
    Volume26
    Issue number6
    DOIs
    StatePublished - Dec 2021

    Bibliographical note

    Funding Information:
    The authors disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: The authors would like to acknowledge the financial support given by MINCIENCIAS (Colombia), Instituto Tecnológico Metropolitano, Universidad de Antioquia, Universidad Pontificia Bolivariana, and Kinetics Systems S.A.S. (Medellín-Colombia), under project number 115077757186.

    Funding Information:
    The authors would like to acknowledge the support given by Dr. Carlos Muskus from PECET Laboratory in Universidad de Antioquia (UdeA), the histopathological laboratory of UdeA, the PECET bioterium Staff (Medellín-Colombia), and Dr. Jorge Arevalo from Universidad Peruana Cayetano Heredia (Lima-Perú). The authors disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: The authors would like to acknowledge the financial support given by MINCIENCIAS (Colombia), Instituto Tecnológico Metropolitano, Universidad de Antioquia, Universidad Pontificia Bolivariana, and Kinetics Systems S.A.S. (Medellín-Colombia), under project number 115077757186.

    Publisher Copyright:
    © Society for Laboratory Automation and Screening 2021.

    Keywords

    • animal model
    • cutaneous leishmaniasis
    • cutaneous ulcers
    • diffuse reflectance
    • histopathologies

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