Diseño de un inmunosensor piezoeléctrico para la detección de biomarcadores de tuberculosis

M. Jaramillo; Y. J. Montagut; A. Montoya; J. Robledo; P. A. Marin; J. E. Betancur; M. J. Moreno; E. Pabon; R. A. Torres

doi:10.1109/PAHCE.2013.6568332

Diseño de un inmunosensor piezoeléctrico para la detección de biomarcadores de tuberculosis

Translated title of the contribution: Design of a piezoelectric immunosensor for tuberculosis biomarker detection

M. Jaramillo, Y. J. Montagut, A. Montoya, J. Robledo, P. A. Marin, J. E. Betancur, M. J. Moreno, E. Pabon, R. A. Torres

Research output: Chapter in Book/Report/Conference proceeding › Conference and proceedings › peer-review

3 Scopus citations

Abstract

Monoclonal antibodies have been applied in biomedical research for diagnosis of various diseases and for the treatment of increasing prevalence diseases such as tuberculosis. There is a need to develop systems capable of detecting tuberculosis-related biomarkers that can provide a quick diagnosis to begin treatment immediately. Thanks to the advances in research for detection, biosensors technologies are emerging as a good alternative for clinical applications. The aim of this work was to develop a piezoelectric immunosensor for rapid and efficient tuberculosis detection. To this purpose, 12 hybridomas and their secreted monoclonal antibodies specific for a biomarker of Mycobacterium tuberculosis were obtained as potential bio-receptors. The monoclonal antibody affinity to the antigenic protein of interest was tested by ELISA. Surface functionalization of the gold electrode - the transducer - of a Quartz Crystal Microbalance (QCM) was simulated by the formation of Self-Assembled Monolayers (SAM) of BSA as a model protein. The functionalized surface was image-characterized by Atomic Force Microscopy (AFM). The appropriate electronic instrumentation system for QCM characterization, with temperature control, was also developed. The obtained immunoreagents will be used as specific recognition bioactive molecules, in combination with functionalized quartz crystals, to build a piezoelectric immunosensor for the detection of Mycobacterium tuberculosis biomarkers, which can be related to the disease.

Translated title of the contribution	Design of a piezoelectric immunosensor for tuberculosis biomarker detection
Original language	Spanish
Title of host publication	2013 Pan American Health Care Exchanges, PAHCE 2013 - Conference, Workshops, and Exhibits. Cooperation / Linkages
Subtitle of host publication	An Independent Forum for Patient Care and Technology Support
DOIs	https://doi.org/10.1109/PAHCE.2013.6568332
State	Published - 2013
Event	8th Pan American Health Care Exchanges Conference, PAHCE 2013 - Medellin, Colombia Duration: 29 Apr 2013 → 4 May 2013

Publication series

Name	Pan American Health Care Exchanges, PAHCE
ISSN (Print)	2327-8161
ISSN (Electronic)	2327-817X

Conference

Conference	8th Pan American Health Care Exchanges Conference, PAHCE 2013
Country/Territory	Colombia
City	Medellin
Period	29/04/13 → 4/05/13

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1109/PAHCE.2013.6568332

Cite this

Jaramillo, M., Montagut, Y. J., Montoya, A., Robledo, J., Marin, P. A., Betancur, J. E., Moreno, M. J., Pabon, E., & Torres, R. A. (2013). Diseño de un inmunosensor piezoeléctrico para la detección de biomarcadores de tuberculosis. In 2013 Pan American Health Care Exchanges, PAHCE 2013 - Conference, Workshops, and Exhibits. Cooperation / Linkages: An Independent Forum for Patient Care and Technology Support Article 6568332 (Pan American Health Care Exchanges, PAHCE). https://doi.org/10.1109/PAHCE.2013.6568332

Jaramillo, M. ; Montagut, Y. J. ; Montoya, A. et al. / Diseño de un inmunosensor piezoeléctrico para la detección de biomarcadores de tuberculosis. 2013 Pan American Health Care Exchanges, PAHCE 2013 - Conference, Workshops, and Exhibits. Cooperation / Linkages: An Independent Forum for Patient Care and Technology Support. 2013. (Pan American Health Care Exchanges, PAHCE).

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title = "Dise{\~n}o de un inmunosensor piezoel{\'e}ctrico para la detecci{\'o}n de biomarcadores de tuberculosis",

abstract = "Monoclonal antibodies have been applied in biomedical research for diagnosis of various diseases and for the treatment of increasing prevalence diseases such as tuberculosis. There is a need to develop systems capable of detecting tuberculosis-related biomarkers that can provide a quick diagnosis to begin treatment immediately. Thanks to the advances in research for detection, biosensors technologies are emerging as a good alternative for clinical applications. The aim of this work was to develop a piezoelectric immunosensor for rapid and efficient tuberculosis detection. To this purpose, 12 hybridomas and their secreted monoclonal antibodies specific for a biomarker of Mycobacterium tuberculosis were obtained as potential bio-receptors. The monoclonal antibody affinity to the antigenic protein of interest was tested by ELISA. Surface functionalization of the gold electrode - the transducer - of a Quartz Crystal Microbalance (QCM) was simulated by the formation of Self-Assembled Monolayers (SAM) of BSA as a model protein. The functionalized surface was image-characterized by Atomic Force Microscopy (AFM). The appropriate electronic instrumentation system for QCM characterization, with temperature control, was also developed. The obtained immunoreagents will be used as specific recognition bioactive molecules, in combination with functionalized quartz crystals, to build a piezoelectric immunosensor for the detection of Mycobacterium tuberculosis biomarkers, which can be related to the disease.",

keywords = "AFM, Mycobacterium tuberculosis, biomarker, monoclonal antiboies, piezoelectric inmunosensor",

author = "M. Jaramillo and Montagut, {Y. J.} and A. Montoya and J. Robledo and Marin, {P. A.} and Betancur, {J. E.} and Moreno, {M. J.} and E. Pabon and Torres, {R. A.}",

year = "2013",

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language = "Espa{\~n}ol",

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series = "Pan American Health Care Exchanges, PAHCE",

booktitle = "2013 Pan American Health Care Exchanges, PAHCE 2013 - Conference, Workshops, and Exhibits. Cooperation / Linkages",

note = "8th Pan American Health Care Exchanges Conference, PAHCE 2013 ; Conference date: 29-04-2013 Through 04-05-2013",

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Jaramillo, M, Montagut, YJ, Montoya, A, Robledo, J, Marin, PA, Betancur, JE, Moreno, MJ, Pabon, E & Torres, RA 2013, Diseño de un inmunosensor piezoeléctrico para la detección de biomarcadores de tuberculosis. in 2013 Pan American Health Care Exchanges, PAHCE 2013 - Conference, Workshops, and Exhibits. Cooperation / Linkages: An Independent Forum for Patient Care and Technology Support., 6568332, Pan American Health Care Exchanges, PAHCE, 8th Pan American Health Care Exchanges Conference, PAHCE 2013, Medellin, Colombia, 29/04/13. https://doi.org/10.1109/PAHCE.2013.6568332

Diseño de un inmunosensor piezoeléctrico para la detección de biomarcadores de tuberculosis. / Jaramillo, M.; Montagut, Y. J.; Montoya, A. et al.
2013 Pan American Health Care Exchanges, PAHCE 2013 - Conference, Workshops, and Exhibits. Cooperation / Linkages: An Independent Forum for Patient Care and Technology Support. 2013. 6568332 (Pan American Health Care Exchanges, PAHCE).

Research output: Chapter in Book/Report/Conference proceeding › Conference and proceedings › peer-review

TY - GEN

T1 - Diseño de un inmunosensor piezoeléctrico para la detección de biomarcadores de tuberculosis

AU - Jaramillo, M.

AU - Montagut, Y. J.

AU - Montoya, A.

AU - Robledo, J.

AU - Marin, P. A.

AU - Betancur, J. E.

AU - Moreno, M. J.

AU - Pabon, E.

AU - Torres, R. A.

PY - 2013

Y1 - 2013

N2 - Monoclonal antibodies have been applied in biomedical research for diagnosis of various diseases and for the treatment of increasing prevalence diseases such as tuberculosis. There is a need to develop systems capable of detecting tuberculosis-related biomarkers that can provide a quick diagnosis to begin treatment immediately. Thanks to the advances in research for detection, biosensors technologies are emerging as a good alternative for clinical applications. The aim of this work was to develop a piezoelectric immunosensor for rapid and efficient tuberculosis detection. To this purpose, 12 hybridomas and their secreted monoclonal antibodies specific for a biomarker of Mycobacterium tuberculosis were obtained as potential bio-receptors. The monoclonal antibody affinity to the antigenic protein of interest was tested by ELISA. Surface functionalization of the gold electrode - the transducer - of a Quartz Crystal Microbalance (QCM) was simulated by the formation of Self-Assembled Monolayers (SAM) of BSA as a model protein. The functionalized surface was image-characterized by Atomic Force Microscopy (AFM). The appropriate electronic instrumentation system for QCM characterization, with temperature control, was also developed. The obtained immunoreagents will be used as specific recognition bioactive molecules, in combination with functionalized quartz crystals, to build a piezoelectric immunosensor for the detection of Mycobacterium tuberculosis biomarkers, which can be related to the disease.

AB - Monoclonal antibodies have been applied in biomedical research for diagnosis of various diseases and for the treatment of increasing prevalence diseases such as tuberculosis. There is a need to develop systems capable of detecting tuberculosis-related biomarkers that can provide a quick diagnosis to begin treatment immediately. Thanks to the advances in research for detection, biosensors technologies are emerging as a good alternative for clinical applications. The aim of this work was to develop a piezoelectric immunosensor for rapid and efficient tuberculosis detection. To this purpose, 12 hybridomas and their secreted monoclonal antibodies specific for a biomarker of Mycobacterium tuberculosis were obtained as potential bio-receptors. The monoclonal antibody affinity to the antigenic protein of interest was tested by ELISA. Surface functionalization of the gold electrode - the transducer - of a Quartz Crystal Microbalance (QCM) was simulated by the formation of Self-Assembled Monolayers (SAM) of BSA as a model protein. The functionalized surface was image-characterized by Atomic Force Microscopy (AFM). The appropriate electronic instrumentation system for QCM characterization, with temperature control, was also developed. The obtained immunoreagents will be used as specific recognition bioactive molecules, in combination with functionalized quartz crystals, to build a piezoelectric immunosensor for the detection of Mycobacterium tuberculosis biomarkers, which can be related to the disease.

KW - AFM

KW - Mycobacterium tuberculosis

KW - biomarker

KW - monoclonal antiboies

KW - piezoelectric inmunosensor

UR - http://www.scopus.com/inward/record.url?scp=84884340366&partnerID=8YFLogxK

U2 - 10.1109/PAHCE.2013.6568332

DO - 10.1109/PAHCE.2013.6568332

M3 - Ponencia publicada en las memorias del evento con ISBN

AN - SCOPUS:84884340366

SN - 9781467362559

T3 - Pan American Health Care Exchanges, PAHCE

BT - 2013 Pan American Health Care Exchanges, PAHCE 2013 - Conference, Workshops, and Exhibits. Cooperation / Linkages

T2 - 8th Pan American Health Care Exchanges Conference, PAHCE 2013

Y2 - 29 April 2013 through 4 May 2013

ER -

Jaramillo M, Montagut YJ, Montoya A, Robledo J, Marin PA, Betancur JE et al. Diseño de un inmunosensor piezoeléctrico para la detección de biomarcadores de tuberculosis. In 2013 Pan American Health Care Exchanges, PAHCE 2013 - Conference, Workshops, and Exhibits. Cooperation / Linkages: An Independent Forum for Patient Care and Technology Support. 2013. 6568332. (Pan American Health Care Exchanges, PAHCE). doi: 10.1109/PAHCE.2013.6568332

Diseño de un inmunosensor piezoeléctrico para la detección de biomarcadores de tuberculosis

Abstract

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UN SDGs

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