Estimation of biological parameters of cutaneous ulcers caused by leishmaniasis in an animal model using diffuse reflectance spectroscopy

Deivid Botina, Ricardo Franco, Javier Murillo, July Galeano, Artur Zarzycki, Maria C. Torres-Madronero, Camilo Bermúdez, Jaime Montaño, Johnson Garzón, Franck Marzani, Sara M. Robledo

    Research output: Contribution to journalArticle in an indexed scientific journalpeer-review

    7 Scopus citations


    Cutaneous leishmaniasis (CL) is a neglected tropical disease that requires novel tools for its understanding, diagnosis, and treatment follow-up. In the cases of other cutaneous pathologies, such as cancer or cutaneous ulcers due to diabetes, optical diffuse reflectance-based tools and methods are widely used for the investigation of those illnesses. These types of tools and methods offer the possibility to develop portable diagnosis and treatment follow-up systems. In this article, we propose the use of a three-layer diffuse reflectance model for the study of the formation of cutaneous ulcers caused by CL. The proposed model together with an inverse-modeling procedure were used in the evaluation of diffuse-reflectance spectral signatures acquired from cutaneous ulcers formed in the dorsal area of 21 golden hamsters inoculated with Leishmanisis braziliensis. As result, the quantification of the model’s variables related to the main biological parameters of skin were obtained, such as: diameter and volumetric fraction of keratinocytes, collagen; volumetric fraction of hemoglobin, and oxygen saturation. Those parameters show statistically significant differences among the different stages of the CL ulcer formation. We found that these differences are coherent with histopathological manifestations reported in the literature for the main phases of CL formation.

    Original languageEnglish
    Article number4674
    JournalSensors (Switzerland)
    Issue number21
    StatePublished - 1 Nov 2019

    Bibliographical note

    Publisher Copyright:
    © 2019 by the authors. Licensee MDPI, Basel, Switzerland.


    • Cutaneous leishmaniasis
    • Cutaneous ulcer
    • Golden hamster
    • Light-tissue interaction model
    • Model inversion

    Types Minciencias

    • Artículos de investigación con calidad A1 / Q1


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