Evaluación del efecto genotóxico y mutagénico en linfocitos humanos expuestos a nanotubos de carbono modificados

Claudia Espinosa; Lina Marcela Hoyos-Palacio; Lucelly López-López; Daniel Gallego-González; Andrés David Aranzazu-Ceballos; Juan Sebastián Marín-Cárdenas; Dayana Andrea Quintero-Moreno; Isabel Cristina Ortiz-Trujillo

doi:10.15446/dyna.v85n205.68767

Evaluación del efecto genotóxico y mutagénico en linfocitos humanos expuestos a nanotubos de carbono modificados

Translated title of the contribution: Evaluation of the genotoxic and mutagenic effect in human lymphocytes exposed to modified carbon nanotubes

Claudia Espinosa, Lina Marcela Hoyos-Palacio, Lucelly López-López, Daniel Gallego-González, Andrés David Aranzazu-Ceballos, Juan Sebastián Marín-Cárdenas, Dayana Andrea Quintero-Moreno, Isabel Cristina Ortiz-Trujillo

Research output: Contribution to journal › Article in an indexed scientific journal › Research › peer-review

Abstract

Objective: evaluate the genotoxic and mutagenic effect in human lymphocytes exposed to pristine and N-doped carbon nanotubes. Methods: Methods: human lymphocytes were exposed to pristine and N-doped carbon nanotubes (NTC) (0.08, 0.09, 0.1mg/mL). Sister chromatid exchange (SCE) and chromosomal alterations (CA) tests were evaluated. Results: chromatid breaks and chromosomal breaks (double strand breaks) were identified in lymphocytes exposed to 0.1 and 0.08 mg/mL of the pristine CNT. N-doped CNT, induced dicentric chromosomes and chromosomal rings. There was significant difference in the percentage of SCE of cells treated with doped NTC versus negative control and pristine NTC (p<0.0001). Conclusion: the highest concentration of pristine CNTs induced the most chromatid and chromosomal damage. Although the percentage was less than 10%, such degree of damage is considered harmful to cells. Lymphocytes treated with N-doped CNT presented lower percentages of CA and high percentage of SCE, which shows a greater repair of genetic material with these compounds.

Translated title of the contribution	Evaluation of the genotoxic and mutagenic effect in human lymphocytes exposed to modified carbon nanotubes
Original language	Spanish
Pages (from-to)	348-354
Number of pages	7
Journal	DYNA (Colombia)
Volume	85
Issue number	205
DOIs	https://doi.org/10.15446/dyna.v85n205.68767
State	Published - 1 Apr 2018

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Publisher Copyright:
© The author; licensee Universidad Nacional de Colombia.

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Espinosa, C., Hoyos-Palacio, L. M., López-López, L., Gallego-González, D., Aranzazu-Ceballos, A. D., Marín-Cárdenas, J. S., Quintero-Moreno, D. A., & Ortiz-Trujillo, I. C. (2018). Evaluación del efecto genotóxico y mutagénico en linfocitos humanos expuestos a nanotubos de carbono modificados. DYNA (Colombia), 85(205), 348-354. https://doi.org/10.15446/dyna.v85n205.68767

@article{d1ad65e91d464cbaa55ccc8738cbec6d,

title = "Evaluaci{\'o}n del efecto genot{\'o}xico y mutag{\'e}nico en linfocitos humanos expuestos a nanotubos de carbono modificados",

abstract = "Objective: evaluate the genotoxic and mutagenic effect in human lymphocytes exposed to pristine and N-doped carbon nanotubes. Methods: Methods: human lymphocytes were exposed to pristine and N-doped carbon nanotubes (NTC) (0.08, 0.09, 0.1mg/mL). Sister chromatid exchange (SCE) and chromosomal alterations (CA) tests were evaluated. Results: chromatid breaks and chromosomal breaks (double strand breaks) were identified in lymphocytes exposed to 0.1 and 0.08 mg/mL of the pristine CNT. N-doped CNT, induced dicentric chromosomes and chromosomal rings. There was significant difference in the percentage of SCE of cells treated with doped NTC versus negative control and pristine NTC (p<0.0001). Conclusion: the highest concentration of pristine CNTs induced the most chromatid and chromosomal damage. Although the percentage was less than 10%, such degree of damage is considered harmful to cells. Lymphocytes treated with N-doped CNT presented lower percentages of CA and high percentage of SCE, which shows a greater repair of genetic material with these compounds.",

keywords = "Carbon nanotubes, Genotoxicity, Lymphocytes, Mutagenicity tests",

author = "Claudia Espinosa and Hoyos-Palacio, {Lina Marcela} and Lucelly L{\'o}pez-L{\'o}pez and Daniel Gallego-Gonz{\'a}lez and Aranzazu-Ceballos, {Andr{\'e}s David} and Mar{\'i}n-C{\'a}rdenas, {Juan Sebasti{\'a}n} and Quintero-Moreno, {Dayana Andrea} and Ortiz-Trujillo, {Isabel Cristina}",

note = "Publisher Copyright: {\textcopyright} The author; licensee Universidad Nacional de Colombia.",

year = "2018",

month = apr,

day = "1",

doi = "10.15446/dyna.v85n205.68767",

language = "Espa{\~n}ol",

volume = "85",

pages = "348--354",

journal = "DYNA (Colombia)",

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publisher = "Universidad Nacional de Colombia",

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Espinosa, C, Hoyos-Palacio, LM , López-López, L, Gallego-González, D, Aranzazu-Ceballos, AD, Marín-Cárdenas, JS, Quintero-Moreno, DA & Ortiz-Trujillo, IC 2018, 'Evaluación del efecto genotóxico y mutagénico en linfocitos humanos expuestos a nanotubos de carbono modificados', DYNA (Colombia), vol. 85, no. 205, pp. 348-354. https://doi.org/10.15446/dyna.v85n205.68767

Evaluación del efecto genotóxico y mutagénico en linfocitos humanos expuestos a nanotubos de carbono modificados. / Espinosa, Claudia; Hoyos-Palacio, Lina Marcela ; López-López, Lucelly et al.
In: DYNA (Colombia), Vol. 85, No. 205, 01.04.2018, p. 348-354.

Research output: Contribution to journal › Article in an indexed scientific journal › Research › peer-review

TY - JOUR

T1 - Evaluación del efecto genotóxico y mutagénico en linfocitos humanos expuestos a nanotubos de carbono modificados

AU - Espinosa, Claudia

AU - Hoyos-Palacio, Lina Marcela

AU - López-López, Lucelly

AU - Gallego-González, Daniel

AU - Aranzazu-Ceballos, Andrés David

AU - Marín-Cárdenas, Juan Sebastián

AU - Quintero-Moreno, Dayana Andrea

AU - Ortiz-Trujillo, Isabel Cristina

PY - 2018/4/1

Y1 - 2018/4/1

N2 - Objective: evaluate the genotoxic and mutagenic effect in human lymphocytes exposed to pristine and N-doped carbon nanotubes. Methods: Methods: human lymphocytes were exposed to pristine and N-doped carbon nanotubes (NTC) (0.08, 0.09, 0.1mg/mL). Sister chromatid exchange (SCE) and chromosomal alterations (CA) tests were evaluated. Results: chromatid breaks and chromosomal breaks (double strand breaks) were identified in lymphocytes exposed to 0.1 and 0.08 mg/mL of the pristine CNT. N-doped CNT, induced dicentric chromosomes and chromosomal rings. There was significant difference in the percentage of SCE of cells treated with doped NTC versus negative control and pristine NTC (p<0.0001). Conclusion: the highest concentration of pristine CNTs induced the most chromatid and chromosomal damage. Although the percentage was less than 10%, such degree of damage is considered harmful to cells. Lymphocytes treated with N-doped CNT presented lower percentages of CA and high percentage of SCE, which shows a greater repair of genetic material with these compounds.

AB - Objective: evaluate the genotoxic and mutagenic effect in human lymphocytes exposed to pristine and N-doped carbon nanotubes. Methods: Methods: human lymphocytes were exposed to pristine and N-doped carbon nanotubes (NTC) (0.08, 0.09, 0.1mg/mL). Sister chromatid exchange (SCE) and chromosomal alterations (CA) tests were evaluated. Results: chromatid breaks and chromosomal breaks (double strand breaks) were identified in lymphocytes exposed to 0.1 and 0.08 mg/mL of the pristine CNT. N-doped CNT, induced dicentric chromosomes and chromosomal rings. There was significant difference in the percentage of SCE of cells treated with doped NTC versus negative control and pristine NTC (p<0.0001). Conclusion: the highest concentration of pristine CNTs induced the most chromatid and chromosomal damage. Although the percentage was less than 10%, such degree of damage is considered harmful to cells. Lymphocytes treated with N-doped CNT presented lower percentages of CA and high percentage of SCE, which shows a greater repair of genetic material with these compounds.

KW - Carbon nanotubes

KW - Genotoxicity

KW - Lymphocytes

KW - Mutagenicity tests

UR - http://www.scopus.com/inward/record.url?scp=85060989313&partnerID=8YFLogxK

U2 - 10.15446/dyna.v85n205.68767

DO - 10.15446/dyna.v85n205.68767

M3 - Artículo en revista científica indexada

AN - SCOPUS:85060989313

SN - 0012-7353

VL - 85

SP - 348

EP - 354

JO - DYNA (Colombia)

JF - DYNA (Colombia)

IS - 205

ER -

Evaluación del efecto genotóxico y mutagénico en linfocitos humanos expuestos a nanotubos de carbono modificados

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