Evaluación del efecto genotóxico y mutagénico en linfocitos humanos expuestos a nanotubos de carbono modificados

Translated title of the contribution: Evaluation of the genotoxic and mutagenic effect in human lymphocytes exposed to modified carbon nanotubes

Claudia Espinosa, Lina Marcela Hoyos-Palacio, Lucelly López-López, Daniel Gallego-González, Andrés David Aranzazu-Ceballos, Juan Sebastián Marín-Cárdenas, Dayana Andrea Quintero-Moreno, Isabel Cristina Ortiz-Trujillo

    Research output: Contribution to journalArticle in an indexed scientific journalpeer-review

    Abstract

    Objective: evaluate the genotoxic and mutagenic effect in human lymphocytes exposed to pristine and N-doped carbon nanotubes. Methods: Methods: human lymphocytes were exposed to pristine and N-doped carbon nanotubes (NTC) (0.08, 0.09, 0.1mg/mL). Sister chromatid exchange (SCE) and chromosomal alterations (CA) tests were evaluated. Results: chromatid breaks and chromosomal breaks (double strand breaks) were identified in lymphocytes exposed to 0.1 and 0.08 mg/mL of the pristine CNT. N-doped CNT, induced dicentric chromosomes and chromosomal rings. There was significant difference in the percentage of SCE of cells treated with doped NTC versus negative control and pristine NTC (p<0.0001). Conclusion: the highest concentration of pristine CNTs induced the most chromatid and chromosomal damage. Although the percentage was less than 10%, such degree of damage is considered harmful to cells. Lymphocytes treated with N-doped CNT presented lower percentages of CA and high percentage of SCE, which shows a greater repair of genetic material with these compounds.

    Translated title of the contributionEvaluation of the genotoxic and mutagenic effect in human lymphocytes exposed to modified carbon nanotubes
    Original languageSpanish
    Pages (from-to)348-354
    Number of pages7
    JournalDYNA (Colombia)
    Volume85
    Issue number205
    DOIs
    StatePublished - 1 Apr 2018

    Bibliographical note

    Publisher Copyright:
    © The author; licensee Universidad Nacional de Colombia.

    Types Minciencias

    • Artículos de investigación con calidad Q3

    Fingerprint

    Dive into the research topics of 'Evaluation of the genotoxic and mutagenic effect in human lymphocytes exposed to modified carbon nanotubes'. Together they form a unique fingerprint.

    Cite this