High transient colonization by Pneumocystis jirovecii between mothers and newborn

Cristian Vera, Yudy A. Aguilar, Lázaro A. Vélez, Zulma Vanessa Rueda

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

The aim of the study was to explore the frequency and dynamics of acquisition and colonization of Pneumocystis jirovecii among neonates, as well as the epidemiological and genotypic characteristics in mother-child binomial. In a prospective enrolled cohort of women in their third trimester of pregnancy, nasopharyngeal swabs (NPS) and clinical and epidemiological data were collected at four different times: 17 days, 2nd, 4th, and 6th month of life of the newborn. P. jirovecii was detected by nested-PCR for the mtLSU-rRNA gene in each NPS; the genotypes were determined amplifying four genes. Forty-three pairs and 301 NPS were included. During the third trimester, 16.3% of pregnant women were colonized. The rate of colonization in mothers at delivery was 16, 6, 16, and 5% and in their children 28, 43, 42, and 25%, respectively. Within pregnant women, 53% remained negative throughout follow-up, and among these, 91% of their children were positive in at least one of their samples. In both, mothers and children, the most frequent genotype of P. jirovecii was 1. Conclusion: The frequency of colonization by P. jirovecii was higher in newborns than in their respective progenitors. Colonization of both mothers and children is transitory; however, the mother of the newborn is not necessarily the source of primary infection.What is Known:• We did not find studies comparing P. jirovecii colonization between mothers and children simultaneously, yet the frequency of colonization by serologic and molecular methods in pregnant women has been reported.What is New:• According to our findings, 3/4 of the children had transient colonization during the first 6 months of life, in only half in the mothers, without proof of mother-to-child transmission or vice versa.

Original languageEnglish
Pages (from-to)1619-1627
Number of pages9
JournalEuropean Journal of Pediatrics
Volume176
Issue number12
DOIs
StatePublished - 1 Dec 2017
Externally publishedYes

Bibliographical note

Funding Information:
Authors’ contributions Substantial contributions to the conception or design of the work: CV, YAA, LAV, and ZVR. The acquisition of data: CV. Analysis and interpretation of data for the work: CV, YAA, LAV, and ZVR. Drafting the work: CVand ZVR. Revising it critically for important intellectual content: YAA and LAV. Final approval of the version to be published: CV, YA, LAV, and ZVR. Agreement to be accountable for all aspects of the work in ensuring that questions related to the accuracy or integrity of any part of the work are appropriately investigated and resolved: CV, YA, LAV, and ZVR.Funding informationThis study was funded by Pedro Nel Cardona Foundation and ISIS Foundation, grant number 523-16. Epidemiology Group at the Universidad of Antioquia supported us with sample sequencing.

Publisher Copyright:
© 2017, Springer-Verlag GmbH Germany.

Keywords

  • Colonization
  • Mother
  • Neonate
  • Pneumocystis jirovecii
  • Primary infection

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