Histopathologic and immunologic effects of the itraconazole treatment in a murine model of chronic pulmonary paracoccidioidomycosis

Tonny W. Naranjo; Damaris E. Lopera; Lucy R. Diaz-Granados; Jhon J. Duque; Angela Restrepo; Luz E. Cano

doi:10.1016/j.micinf.2010.07.013

Histopathologic and immunologic effects of the itraconazole treatment in a murine model of chronic pulmonary paracoccidioidomycosis

Tonny W. Naranjo, Damaris E. Lopera, Lucy R. Diaz-Granados, Jhon J. Duque, Angela Restrepo, Luz E. Cano

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

A comparative study, based on histopathologic findings (inflammation, cellularity, and fibrosis) and immunologic parameters (pro-inflammatory and anti-inflammatory cytokines), was carried out in order to evaluate the effects of itraconazole (ITC) treatment and its starting time in a BALB/c murine model of chronic pulmonary paracoccidioidomycosis (PCM), induced by intranasal inoculation of Paracoccidioides brasiliensis (Pb) conidia. Two different groups of mice were exposed to ITC therapy beginning at the 4th or 8th week after Pb infection, respectively. ITC was administered daily, via gavage, for a period of sixty days. At weeks 0, 4, 8, 12 and 16 the animals were sacrificed and their lungs removed for histology staining with hematoxylin and eosin (H&E), Masson's trichromic and Gomori-Grocott; pulmonary levels of IL-1β, TNF-α, IFN-γ, IL-13 and TGF-β were also measured by ELISA. The development or absence of the principal pulmonary PCM sequela, lung fibrosis, was directly related to the therapy's starting time. This and other histopathologic findings were related to the behavior of cytokine levels.

Original language	English
Pages (from-to)	1153-1162
Number of pages	10
Journal	Microbes and Infection
Volume	12
Issue number	14-15
DOIs	https://doi.org/10.1016/j.micinf.2010.07.013
State	Published - Dec 2010

Bibliographical note

Funding Information:
This work was supported by COLCIENCIAS (project No. 2213-04-16439 ), Bogotá, Colombia, by the Corporación para Investigaciones Biológicas (CIB) and by the University of Antioquia (UdeA) , Medellín, Colombia.

Keywords

Cytokines
Itraconazole
Lung fibrosis
Paracoccidioidomycosis

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.micinf.2010.07.013

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title = "Histopathologic and immunologic effects of the itraconazole treatment in a murine model of chronic pulmonary paracoccidioidomycosis",

abstract = "A comparative study, based on histopathologic findings (inflammation, cellularity, and fibrosis) and immunologic parameters (pro-inflammatory and anti-inflammatory cytokines), was carried out in order to evaluate the effects of itraconazole (ITC) treatment and its starting time in a BALB/c murine model of chronic pulmonary paracoccidioidomycosis (PCM), induced by intranasal inoculation of Paracoccidioides brasiliensis (Pb) conidia. Two different groups of mice were exposed to ITC therapy beginning at the 4th or 8th week after Pb infection, respectively. ITC was administered daily, via gavage, for a period of sixty days. At weeks 0, 4, 8, 12 and 16 the animals were sacrificed and their lungs removed for histology staining with hematoxylin and eosin (H&E), Masson's trichromic and Gomori-Grocott; pulmonary levels of IL-1β, TNF-α, IFN-γ, IL-13 and TGF-β were also measured by ELISA. The development or absence of the principal pulmonary PCM sequela, lung fibrosis, was directly related to the therapy's starting time. This and other histopathologic findings were related to the behavior of cytokine levels.",

keywords = "Cytokines, Itraconazole, Lung fibrosis, Paracoccidioidomycosis",

author = "Naranjo, {Tonny W.} and Lopera, {Damaris E.} and Diaz-Granados, {Lucy R.} and Duque, {Jhon J.} and Angela Restrepo and Cano, {Luz E.}",

note = "Funding Information: This work was supported by COLCIENCIAS (project No. 2213-04-16439 ), Bogot{\'a}, Colombia, by the Corporaci{\'o}n para Investigaciones Biol{\'o}gicas (CIB) and by the University of Antioquia (UdeA) , Medell{\'i}n, Colombia.",

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AU - Lopera, Damaris E.

AU - Diaz-Granados, Lucy R.

AU - Duque, Jhon J.

AU - Restrepo, Angela

AU - Cano, Luz E.

N1 - Funding Information: This work was supported by COLCIENCIAS (project No. 2213-04-16439 ), Bogotá, Colombia, by the Corporación para Investigaciones Biológicas (CIB) and by the University of Antioquia (UdeA) , Medellín, Colombia.

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N2 - A comparative study, based on histopathologic findings (inflammation, cellularity, and fibrosis) and immunologic parameters (pro-inflammatory and anti-inflammatory cytokines), was carried out in order to evaluate the effects of itraconazole (ITC) treatment and its starting time in a BALB/c murine model of chronic pulmonary paracoccidioidomycosis (PCM), induced by intranasal inoculation of Paracoccidioides brasiliensis (Pb) conidia. Two different groups of mice were exposed to ITC therapy beginning at the 4th or 8th week after Pb infection, respectively. ITC was administered daily, via gavage, for a period of sixty days. At weeks 0, 4, 8, 12 and 16 the animals were sacrificed and their lungs removed for histology staining with hematoxylin and eosin (H&E), Masson's trichromic and Gomori-Grocott; pulmonary levels of IL-1β, TNF-α, IFN-γ, IL-13 and TGF-β were also measured by ELISA. The development or absence of the principal pulmonary PCM sequela, lung fibrosis, was directly related to the therapy's starting time. This and other histopathologic findings were related to the behavior of cytokine levels.

AB - A comparative study, based on histopathologic findings (inflammation, cellularity, and fibrosis) and immunologic parameters (pro-inflammatory and anti-inflammatory cytokines), was carried out in order to evaluate the effects of itraconazole (ITC) treatment and its starting time in a BALB/c murine model of chronic pulmonary paracoccidioidomycosis (PCM), induced by intranasal inoculation of Paracoccidioides brasiliensis (Pb) conidia. Two different groups of mice were exposed to ITC therapy beginning at the 4th or 8th week after Pb infection, respectively. ITC was administered daily, via gavage, for a period of sixty days. At weeks 0, 4, 8, 12 and 16 the animals were sacrificed and their lungs removed for histology staining with hematoxylin and eosin (H&E), Masson's trichromic and Gomori-Grocott; pulmonary levels of IL-1β, TNF-α, IFN-γ, IL-13 and TGF-β were also measured by ELISA. The development or absence of the principal pulmonary PCM sequela, lung fibrosis, was directly related to the therapy's starting time. This and other histopathologic findings were related to the behavior of cytokine levels.

KW - Cytokines

KW - Itraconazole

KW - Lung fibrosis

KW - Paracoccidioidomycosis

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JO - Microbes and Infection

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IS - 14-15

ER -

Histopathologic and immunologic effects of the itraconazole treatment in a murine model of chronic pulmonary paracoccidioidomycosis

Abstract

Bibliographical note

Keywords

UN SDGs

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