Modeling of microbial growth and ammonia consumption at different temperatures in the production of a polyhydroxyalkanoate (PHA) biopolymer

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    Abstract

    Modeling of microbial growth and ammonia consumption at different temperatures was developed in the production of a polyhydroxyalkanoate (PHA) biopolymer in Pseudomona fluorescens in the range of 25-35 °C. A logistic model was employed to predict accurately the microbial growth limiting conditions of nitrogen. A new model based in a mixed mathematical equation comprising a logistic model, and a magnetic saturation model resulted appropriate to estimate the ammonia consumption under limiting conditions. Favorable conditions for PHA production in P. fluorescens were found at temperature of 30 °C, reaching the maximum biomass concentration of 2.83 g L-1, and consuming 99.9% of the initial ammonia, to produce 2.13 g L-1 of PHA. The proposed models could be useful to predict the behavior of a fermentation process to produce PHA in real time.

    Original languageEnglish
    Pages (from-to)498-503
    Number of pages6
    JournalJournal of Applied Research and Technology
    Volume13
    Issue number5
    DOIs
    StatePublished - 2015

    Bibliographical note

    Publisher Copyright:
    © 2015 Universidad Nacional Autónoma de México, Centro de Ciencias Aplicadas y Desarrollo Tecnológico.

    Keywords

    • Ammonia consumption
    • Microbial growth
    • Modeling
    • PHA
    • Temperature effect

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