Optimization of a DNA extraction procedure for anopheline mosquitoes

Doris A. Rosero; Lina A. Gutiérrez; Astrid V. Cienfuegos; Luz M. Jaramillo; Margarita M. Correa

Optimization of a DNA extraction procedure for anopheline mosquitoes

Translated title of the contribution: Optimization of a DNA extraction procedure for anopheline mosquitoes

Doris A. Rosero, Lina A. Gutiérrez, Astrid V. Cienfuegos, Luz M. Jaramillo, Margarita M. Correa

Research output: Contribution to journal › Article in an indexed scientific journal › peer-review

31 Scopus citations

Abstract

A DNA extraction protocol for anopheline mosquitoes was optimized by the evaluation of various incubation times of the sample with Potassium acetate (KAc). The results showed that the highest DNA concentration and amplification frequency were obtained with the 1 h treatment, which suggests that is possible to reduce the incubation time with KAc without affecting the amount and quality of the DNA extracted.

Translated title of the contribution	Optimization of a DNA extraction procedure for anopheline mosquitoes
Original language	English
Pages (from-to)	260-263
Number of pages	4
Journal	Revista Colombiana de Entomologia
Volume	36
Issue number	2
State	Published - Jul 2010
Externally published	Yes

Keywords

Anopheles
PCR
Potassium Acetate

Cite this

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title = "Optimization of a DNA extraction procedure for anopheline mosquitoes",

abstract = "A DNA extraction protocol for anopheline mosquitoes was optimized by the evaluation of various incubation times of the sample with Potassium acetate (KAc). The results showed that the highest DNA concentration and amplification frequency were obtained with the 1 h treatment, which suggests that is possible to reduce the incubation time with KAc without affecting the amount and quality of the DNA extracted.",

keywords = "Anopheles, PCR, Potassium Acetate",

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AU - Rosero, Doris A.

AU - Gutiérrez, Lina A.

AU - Cienfuegos, Astrid V.

AU - Jaramillo, Luz M.

AU - Correa, Margarita M.

PY - 2010/7

Y1 - 2010/7

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AB - A DNA extraction protocol for anopheline mosquitoes was optimized by the evaluation of various incubation times of the sample with Potassium acetate (KAc). The results showed that the highest DNA concentration and amplification frequency were obtained with the 1 h treatment, which suggests that is possible to reduce the incubation time with KAc without affecting the amount and quality of the DNA extracted.

KW - Anopheles

KW - PCR

KW - Potassium Acetate

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M3 - Artículo en revista científica indexada

AN - SCOPUS:79960482794

SN - 0120-0488

VL - 36

SP - 260

EP - 263

JO - Revista Colombiana de Entomologia

JF - Revista Colombiana de Entomologia

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ER -

Optimization of a DNA extraction procedure for anopheline mosquitoes

Abstract

Keywords

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