Optimization of a DNA extraction procedure for anopheline mosquitoes

Translated title of the contribution: Optimization of a DNA extraction procedure for anopheline mosquitoes

Doris A. Rosero, Lina A. Gutiérrez, Astrid V. Cienfuegos, Luz M. Jaramillo, Margarita M. Correa

Research output: Contribution to journalArticle in an indexed scientific journalpeer-review

29 Scopus citations

Abstract

A DNA extraction protocol for anopheline mosquitoes was optimized by the evaluation of various incubation times of the sample with Potassium acetate (KAc). The results showed that the highest DNA concentration and amplification frequency were obtained with the 1 h treatment, which suggests that is possible to reduce the incubation time with KAc without affecting the amount and quality of the DNA extracted.

Translated title of the contributionOptimization of a DNA extraction procedure for anopheline mosquitoes
Original languageEnglish
Pages (from-to)260-263
Number of pages4
JournalRevista Colombiana de Entomologia
Volume36
Issue number2
StatePublished - Jul 2010
Externally publishedYes

Keywords

  • Anopheles
  • PCR
  • Potassium Acetate

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