Abstract
Mycobacterium tuberculosis (MTB) is known for its adaptive capability in developing resistance to antibiotics, through the selection of spontaneous mutations that arise during treatment. Generating spontaneous antibiotic-resistant mutants in vitro is challenging but necessary for studying this phenomenon. A protocol was designed and tested to select stable, MTB spontaneous, d-cycloserine (DCS) resistant mutants. Twenty-four colonies resistant to DCS were selected, demonstrating an increase between 1 and 4 times the Minimum Inhibitory Concentration (MIC) set for Mycobacterium tuberculosis H37Rv ATCC 27294 reference strain.
| Original language | English |
|---|---|
| Article number | 102690 |
| Journal | MethodsX |
| Volume | 12 |
| DOIs | |
| State | Published - Jun 2024 |
Bibliographical note
Publisher Copyright:© 2024
UN SDGs
This output contributes to the following UN Sustainable Development Goals (SDGs)
-
SDG 3 Good Health and Well-being
Keywords
- Antibiotics
- Drug-resistant
- H37Rv
- Mycobacterium tuberculosis
- Spontaneous mutants
Types Minciencias
- Artículos de investigación con calidad A2 / Q2
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