Purificación del antígeno 38 kDa de Mycobacterium tuberculosisy su potencial uso en diagnóstico mediante inmunosensores piezoeléctricos

Translated title of the contribution: Mycobacterium tuberculosis 38 kDa antigen purification and potential diagnostic use by piezoelectric immunosensors

Paula A. Marín, Luz E. Botero, Jaime A. Robledo, Ana M. Murillo, Robinson A. Torres, Yeison J. Montagut, Elizabeth Pabón, Marisol Jaramillo

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

The selection and procurance of the immunoreagents used in the immunoassay and biofunctionalisation transducer strategy, are a key in the piezoelectric immunosensor development for the detection of tuberculosis (TB). Many have reported the use of 38kDa protein antigen (Ag38kDa) fromMycobacterium tuberculosis (Mtb) such as good biomarker of TB disease and compliance with physical and biochemical characteristics to be immobilized by self-assembled monolayers (SAMs), in the gold electrode of piezoelectrics crystals surfaces. A piezoelectric immunosensor developed from purified native antigens of Mtb may be an alternative simple method for detection of Mtb with speed and reusable advantages, contributing to the control and early treatment of disease. In this paper, the purification process of Ag38kDa Mtb from secretory proteins filtered culture (CFP) from Mtb is presented as an immunoreactive with potential application in the detection of Mtb by piezoelectric immunosensors. Functionalized crystals were obtained by using the modified self-assembled monolayers (SAMs) technique, with purified native antigen and CFP. The functionalized surfaces were qualitatively characterized using atomic force microscopy (AFM) in order to validate the immobilization protocol optimal conditions for secretion antigens from Mtb. These modified crystals may be coupled to piezoelectric immunosensor characterization system for detecting of Mtb by a direct competition immunoassay.

Translated title of the contributionMycobacterium tuberculosis 38 kDa antigen purification and potential diagnostic use by piezoelectric immunosensors
Original languageSpanish
Pages (from-to)129-139
Number of pages11
JournalActa Biologica Colombiana
Volume20
Issue number1
DOIs
StatePublished - 2015
Externally publishedYes

Bibliographical note

Publisher Copyright:
© 2015, Universidad Nacional de Colombia. All rights reserved.

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