Abstract
Cryptosporidium parvum and C. hominis are the most relevant species of this genus for human health. Both cause a self-limiting diarrhea in immunocompetent individuals, but cause potentially life-threatening disease in the immunocompromised. Despite the importance of these pathogens, only one reference genome of each has been analyzed and published. These two reference genomes were sequenced using automated capillary sequencing; as of yet, no next generation sequencing technology has been applied to improve their assemblies and annotations. For C. hominis, the main challenge that prevents a larger number of genomes to be sequenced is its resistance to axenic culture. In the present study, we employed next generation technology to analyse the genomic DNA and RNA to generate a new reference genome sequence of a C. hominis strain isolated directly from human stool and a new genome annotation of the C. parvum Iowa reference genome.
Original language | English |
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Article number | 16324 |
Journal | Scientific Reports |
Volume | 5 |
DOIs | |
State | Published - 9 Nov 2015 |
Externally published | Yes |
Bibliographical note
Funding Information:The authors gratefully acknowledge Colciencias (Instituto Colombiano para el Desarrollo de la Ciencia y la Tecnología) for the financial support under the Grant N° 111551928843, San Vicente Fundación Hospital for the contribution of stool samples and Jorge Duitama for his kind assistance using NGSEP package.