Revisiting the reference genomes of human pathogenic Cryptosporidium species: Reannotation of C. parvum Iowa and a new C. hominis reference

Juan P. Isaza, Ana Luz Galvan, Victor Polanco, Bernice Huang, Andrey V. Matveyev, Myrna G. Serrano, Patricio Manque, Gregory A. Buck, Juan F. Alzate

Research output: Contribution to journalArticlepeer-review

37 Scopus citations

Abstract

Cryptosporidium parvum and C. hominis are the most relevant species of this genus for human health. Both cause a self-limiting diarrhea in immunocompetent individuals, but cause potentially life-threatening disease in the immunocompromised. Despite the importance of these pathogens, only one reference genome of each has been analyzed and published. These two reference genomes were sequenced using automated capillary sequencing; as of yet, no next generation sequencing technology has been applied to improve their assemblies and annotations. For C. hominis, the main challenge that prevents a larger number of genomes to be sequenced is its resistance to axenic culture. In the present study, we employed next generation technology to analyse the genomic DNA and RNA to generate a new reference genome sequence of a C. hominis strain isolated directly from human stool and a new genome annotation of the C. parvum Iowa reference genome.

Original languageEnglish
Article number16324
JournalScientific Reports
Volume5
DOIs
StatePublished - 9 Nov 2015
Externally publishedYes

Bibliographical note

Funding Information:
The authors gratefully acknowledge Colciencias (Instituto Colombiano para el Desarrollo de la Ciencia y la Tecnología) for the financial support under the Grant N° 111551928843, San Vicente Fundación Hospital for the contribution of stool samples and Jorge Duitama for his kind assistance using NGSEP package.

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