Techniques of Fibroblasts and Cardiomyocytes Co-Cultures for Atrial Fibrillation Studies

Álvaro Ayazo Patiño, Henry Hermel Andrade Caicedo, Valentina Nieto Marín, Isabel Cristina Ortiz Trujillo

Research output: Contribution to journalArticle in an indexed scientific journalpeer-review

Abstract

Background and Objective: Currently, heart diseases like cardiac arrhythmias are a high public health impact worldwide. One of the most frequent cardiac arrhythmias is atrial fibrillation (AF), a heart condition that causes an irregular and often abnormally fast heart rate. The AF had been linked with atrial fibrosis also, but the cause-effect relationship is not clear yet. This study aimed to simulate the conditions of interaction in atrial fibrosis, through different co-cultured proportions of NIH-3T3 rat fibrotic cells (fibroblasts) and HL-1 rat cardiomyocytes cells.

Materials and Methods: Electrical activity in the cultures was then assessed by optical mapping to study the patterns of action potential propagation between cardiomyocytes, using the voltage-sensitive marker Di-8-ANEPPS.

Results: The cultures were found to be distinguishable within the plates, according to dot and linear patterns performed by parafilm patterns.

Conclusion: Cardiomyocyte’s electrical activity could not be detected in co-cultures, which is necessary to establish the effect of the presence of fibroblasts, whereby studies should be performed with other conditions and measurements to establish possible changes in the patterns.
Original languageSpanish (Colombia)
JournalRJC - Research Journal of Cardiology
Volume15
Issue number1
StatePublished - 1 Jan 2022

Types Minciencias

  • Outreach articles

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