TY - JOUR
T1 - Cytotoxic, mutagenic and genotoxic evaluation of crude extracts and fractions from Piper jericoense with trypanocidal action
AU - Hamedt, A. L.
AU - Ortiz, I. C.
AU - García-Huertas, P. A.
AU - Sáenz, J.
AU - Araujo, A. Caldeira de
AU - De Mattos, J. C.P.
AU - Rodríguez-Gazquez, M. A.
AU - Triana-Chávez, O.
N1 - Funding Information:
This study was supported by Colciencias-Colombia (project 111545921472), and Estrategia de Sostenibilidad 2013-2014 CODI, Universidad de Antioquia.
PY - 2014/3
Y1 - 2014/3
N2 - The current Chagas disease treatment is based on two drugs, nifurtimox and benznidazole, which is considered unsatisfactory, not only because of the narrow therapeutic range but also because of the associated toxicity. Natural products are considered an important source of biologically active compounds against various infectious organisms. Numerous Piper species are used in traditional medicine to treat parasitic diseases. In this paper, we study the activity of extracts and fractions obtained from Piper jericoense plant against epimastigote, trypomastigote and amastigote forms of Trypanosoma cruzi. In addition, we evaluated the cytotoxic, mutagenic and genotoxic activities of the F4 fraction obtained from one of the more promising extracts. We obtained four extracts, one of which presented low toxicity and high trypanocidal activity. This extract was separated into eight fractions, and the F4 fraction presented better results than the other extracts and had a higher selectivity index than the reference drug, benznidazole. This fraction was not cytotoxic, mutagenic or genotoxic.
AB - The current Chagas disease treatment is based on two drugs, nifurtimox and benznidazole, which is considered unsatisfactory, not only because of the narrow therapeutic range but also because of the associated toxicity. Natural products are considered an important source of biologically active compounds against various infectious organisms. Numerous Piper species are used in traditional medicine to treat parasitic diseases. In this paper, we study the activity of extracts and fractions obtained from Piper jericoense plant against epimastigote, trypomastigote and amastigote forms of Trypanosoma cruzi. In addition, we evaluated the cytotoxic, mutagenic and genotoxic activities of the F4 fraction obtained from one of the more promising extracts. We obtained four extracts, one of which presented low toxicity and high trypanocidal activity. This extract was separated into eight fractions, and the F4 fraction presented better results than the other extracts and had a higher selectivity index than the reference drug, benznidazole. This fraction was not cytotoxic, mutagenic or genotoxic.
KW - Antiprotozoal
KW - Chagas
KW - Chemotherapy
KW - Cytotoxicity
KW - Genotoxicity
KW - Mutagenesis
UR - http://www.scopus.com/inward/record.url?scp=84891676749&partnerID=8YFLogxK
U2 - 10.1016/j.actatropica.2013.12.008
DO - 10.1016/j.actatropica.2013.12.008
M3 - Artículo
C2 - 24361641
AN - SCOPUS:84891676749
VL - 131
SP - 92
EP - 97
JO - Acta Tropica
JF - Acta Tropica
SN - 0001-706X
IS - 1
ER -
