Development of a Platform of Cellular Interaction in Tissue Patterns: In Vitro Model of Endomycardial Fibrosis

P. Orozco, Y. Montoya, J. Bustamante

Producción científica: Capítulo del libro/informe/acta de congresoPonencia publicada en las memorias del evento con ISBNrevisión exhaustiva

Resumen

Cardiac arrhythmias and conduction disorders constitute an important group within cardiovascular diseases. Cardiac arrhythmias are derived from multiple factors, including fibrosis in which multifactorial processes are involved as a result of complex cellular interactions. The purpose of this study was to develop a platform for evaluating cellular interactions in tissue patterns to replicating structural behaviors of endomyocardial fibrosis in irregular and compact circular microarchitectures in an in vitro model of cardiomyocytes and fibroblasts. For this research, pattern geometries were designed and modeled emulating the tissue distribution of the circular compact and irregular of endomyocardial fibrosis using Solid Edge and Corel Draw software, and then they were used as molds to generate inserts of Parafilm, later the cell patterning technique was implemented in the in vitro model of cardiomyocytes and fibroblasts emulating endomyocardial fibrosis. Finally, cocultures were stained with hematoxylin-eosin and observed by light field microscopy. In this investigation, it was found that the developed platform allowed the generation of cellular patterns that replicated endomyocardial fibrosis microarchitectures, in which morphological changes were evidenced after the interaction of the RL-14 cardiomyocytes with the 3T3 fibroblasts in the in vitro model; allowing to evaluate biological characteristics such as, proliferation and spatial distribution.

Idioma originalInglés
Título de la publicación alojada2019 Global Medical Engineering Physics Exchanges/ Pan American Health Care Exchanges, GMEPE/PAHCE 2019
EditorialIEEE Computer Society
ISBN (versión digital)9781728100357
DOI
EstadoPublicada - 16 may. 2019
Evento2019 Global Medical Engineering Physics Exchanges/ Pan American Health Care Exchanges, GMEPE/PAHCE 2019 - Buenos Aires, Argentina
Duración: 26 mar. 201931 mar. 2019

Serie de la publicación

NombrePan American Health Care Exchanges, PAHCE
Volumen2019-March
ISSN (versión impresa)2327-8161
ISSN (versión digital)2327-817X

Conferencia

Conferencia2019 Global Medical Engineering Physics Exchanges/ Pan American Health Care Exchanges, GMEPE/PAHCE 2019
País/TerritorioArgentina
CiudadBuenos Aires
Período26/03/1931/03/19

Nota bibliográfica

Publisher Copyright:
© 2019 IEEE.

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