In vitro permeability, porosity and cell growth study in electrospin membranes for vascular grafts

Título traducido de la contribución: Estudio in vitro de permeabilidad, porosidad y crecimiento celular en membranas electrohiladas para prótesis vasculares

Raúl A. Valencia, Yuliet Montoya, Simón Sánchez, Isabel C. Ortiz, Lina M. Hoyos, John Bustamante

    Producción científica: Contribución a una revistaArtículo en revista científica indexadarevisión exhaustiva

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    Resumen

    Introduction and motivation Nowadays, the possibility of engineering vascular grafts by means of electrospinning has attracted interest for small-diameter blood vessels. Potential vascular application lies in their ability to generate microporous structures with a big surface area. However, little is know about the effect of such microstructure and its thickness, both regarding mass transportation as well as cell proliferation. The motivation of this work is to analyse the permeability, porosity and cell growth in electrospun membranes for vascular grafts. Methods by electrospinning membranes for grafts made of memory polyurethane foam were fabricated in two thicknesses, sample 0 between 0.2 and 0.9 mm and sample +1 between 0.9 and 1.0 mm, amongst which human cardiac fibroblasts were grown during an incubation period of up to 10 days. Porosity and permeability were assessed according to ISO 7198 and cell proliferation was analysed with scanning optical and electronic microscopy (SEM). Total sample number was 12 for each baseline thickness. Data analysis was conducted by ANOVA test. Results +1 samples presented an average permeability that was 55.5% lower than the 0 samples, 10.24% decrease in porosity associated to bigger cell growth, evidenced by syncytial cells. Conclusion The variation of microporous structures of great surface area in order to obtain grafts by means of electrospinning technique affects cell growth and subsequently their permeability and porosity, thus offering an opportunity for its potential use in small-diameter vascular applications.

    Título traducido de la contribuciónEstudio in vitro de permeabilidad, porosidad y crecimiento celular en membranas electrohiladas para prótesis vasculares
    Idioma originalInglés
    Páginas (desde-hasta)182-190
    Número de páginas9
    PublicaciónRevista Colombiana de Cardiologia
    Volumen24
    N.º2
    DOI
    EstadoPublicada - 1 mar. 2017

    Nota bibliográfica

    Publisher Copyright:
    © 2016 Sociedad Colombiana de Cardiología y Cirugía Cardiovascular

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