Evaluación del efecto genotóxico y mutagénico en linfocitos humanos expuestos a nanotubos de carbono modificados

Claudia Espinosa, Lina Marcela Hoyos-Palacio, Lucelly López-López, Daniel Gallego-González, Andrés David Aranzazu-Ceballos, Juan Sebastián Marín-Cárdenas, Dayana Andrea Quintero-Moreno, Isabel Cristina Ortiz-Trujillo

    Producción científica: Contribución a una revistaArtículo en revista científica indexadarevisión exhaustiva

    Resumen

    Objective: evaluate the genotoxic and mutagenic effect in human lymphocytes exposed to pristine and N-doped carbon nanotubes. Methods: Methods: human lymphocytes were exposed to pristine and N-doped carbon nanotubes (NTC) (0.08, 0.09, 0.1mg/mL). Sister chromatid exchange (SCE) and chromosomal alterations (CA) tests were evaluated. Results: chromatid breaks and chromosomal breaks (double strand breaks) were identified in lymphocytes exposed to 0.1 and 0.08 mg/mL of the pristine CNT. N-doped CNT, induced dicentric chromosomes and chromosomal rings. There was significant difference in the percentage of SCE of cells treated with doped NTC versus negative control and pristine NTC (p<0.0001). Conclusion: the highest concentration of pristine CNTs induced the most chromatid and chromosomal damage. Although the percentage was less than 10%, such degree of damage is considered harmful to cells. Lymphocytes treated with N-doped CNT presented lower percentages of CA and high percentage of SCE, which shows a greater repair of genetic material with these compounds.

    Título traducido de la contribuciónEvaluation of the genotoxic and mutagenic effect in human lymphocytes exposed to modified carbon nanotubes
    Idioma originalEspañol
    Páginas (desde-hasta)348-354
    Número de páginas7
    PublicaciónDYNA (Colombia)
    Volumen85
    N.º205
    DOI
    EstadoPublicada - 1 abr. 2018

    Nota bibliográfica

    Publisher Copyright:
    © The author; licensee Universidad Nacional de Colombia.

    Palabras clave

    • Carbon nanotubes
    • Genotoxicity
    • Lymphocytes
    • Mutagenicity tests

    Tipos de Productos Minciencias

    • Artículos de investigación con calidad Q3

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