Resumen
Objective: evaluate the genotoxic and mutagenic effect in human lymphocytes exposed to pristine and N-doped carbon nanotubes. Methods: Methods: human lymphocytes were exposed to pristine and N-doped carbon nanotubes (NTC) (0.08, 0.09, 0.1mg/mL). Sister chromatid exchange (SCE) and chromosomal alterations (CA) tests were evaluated. Results: chromatid breaks and chromosomal breaks (double strand breaks) were identified in lymphocytes exposed to 0.1 and 0.08 mg/mL of the pristine CNT. N-doped CNT, induced dicentric chromosomes and chromosomal rings. There was significant difference in the percentage of SCE of cells treated with doped NTC versus negative control and pristine NTC (p<0.0001). Conclusion: the highest concentration of pristine CNTs induced the most chromatid and chromosomal damage. Although the percentage was less than 10%, such degree of damage is considered harmful to cells. Lymphocytes treated with N-doped CNT presented lower percentages of CA and high percentage of SCE, which shows a greater repair of genetic material with these compounds.
Título traducido de la contribución | Evaluation of the genotoxic and mutagenic effect in human lymphocytes exposed to modified carbon nanotubes |
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Idioma original | Español |
Páginas (desde-hasta) | 348-354 |
Número de páginas | 7 |
Publicación | DYNA (Colombia) |
Volumen | 85 |
N.º | 205 |
DOI | |
Estado | Publicada - 1 abr. 2018 |
Nota bibliográfica
Publisher Copyright:© The author; licensee Universidad Nacional de Colombia.
Palabras clave
- Carbon nanotubes
- Genotoxicity
- Lymphocytes
- Mutagenicity tests