TY - JOUR
T1 - Histopathologic and immunologic effects of the itraconazole treatment in a murine model of chronic pulmonary paracoccidioidomycosis
AU - Naranjo, Tonny W.
AU - Lopera, Damaris E.
AU - Diaz-Granados, Lucy R.
AU - Duque, Jhon J.
AU - Restrepo, Angela
AU - Cano, Luz E.
N1 - Funding Information:
This work was supported by COLCIENCIAS (project No. 2213-04-16439 ), Bogotá, Colombia, by the Corporación para Investigaciones Biológicas (CIB) and by the University of Antioquia (UdeA) , Medellín, Colombia.
PY - 2010/12
Y1 - 2010/12
N2 - A comparative study, based on histopathologic findings (inflammation, cellularity, and fibrosis) and immunologic parameters (pro-inflammatory and anti-inflammatory cytokines), was carried out in order to evaluate the effects of itraconazole (ITC) treatment and its starting time in a BALB/c murine model of chronic pulmonary paracoccidioidomycosis (PCM), induced by intranasal inoculation of Paracoccidioides brasiliensis (Pb) conidia. Two different groups of mice were exposed to ITC therapy beginning at the 4th or 8th week after Pb infection, respectively. ITC was administered daily, via gavage, for a period of sixty days. At weeks 0, 4, 8, 12 and 16 the animals were sacrificed and their lungs removed for histology staining with hematoxylin and eosin (H&E), Masson's trichromic and Gomori-Grocott; pulmonary levels of IL-1β, TNF-α, IFN-γ, IL-13 and TGF-β were also measured by ELISA. The development or absence of the principal pulmonary PCM sequela, lung fibrosis, was directly related to the therapy's starting time. This and other histopathologic findings were related to the behavior of cytokine levels.
AB - A comparative study, based on histopathologic findings (inflammation, cellularity, and fibrosis) and immunologic parameters (pro-inflammatory and anti-inflammatory cytokines), was carried out in order to evaluate the effects of itraconazole (ITC) treatment and its starting time in a BALB/c murine model of chronic pulmonary paracoccidioidomycosis (PCM), induced by intranasal inoculation of Paracoccidioides brasiliensis (Pb) conidia. Two different groups of mice were exposed to ITC therapy beginning at the 4th or 8th week after Pb infection, respectively. ITC was administered daily, via gavage, for a period of sixty days. At weeks 0, 4, 8, 12 and 16 the animals were sacrificed and their lungs removed for histology staining with hematoxylin and eosin (H&E), Masson's trichromic and Gomori-Grocott; pulmonary levels of IL-1β, TNF-α, IFN-γ, IL-13 and TGF-β were also measured by ELISA. The development or absence of the principal pulmonary PCM sequela, lung fibrosis, was directly related to the therapy's starting time. This and other histopathologic findings were related to the behavior of cytokine levels.
KW - Cytokines
KW - Itraconazole
KW - Lung fibrosis
KW - Paracoccidioidomycosis
UR - http://www.scopus.com/inward/record.url?scp=78649898793&partnerID=8YFLogxK
U2 - 10.1016/j.micinf.2010.07.013
DO - 10.1016/j.micinf.2010.07.013
M3 - Artículo en revista científica indexada
C2 - 20691804
AN - SCOPUS:78649898793
SN - 1286-4579
VL - 12
SP - 1153
EP - 1162
JO - Microbes and Infection
JF - Microbes and Infection
IS - 14-15
ER -
