In vitro and in vivo antileishmanial activity of Artemisia annua L. Leaf powder and its potential usefulness in the treatment of uncomplicated cutaneous leishmaniasis in humans

Luz Estella Mesa, Daniel Vasquez, Pierre Lutgen, Iván Darío Vélez, Adriana María Restrepo, Isabel Ortiz, Sara María Robledo

    Producción científica: Contribución a una revistaArtículo en revista científica indexadarevisión exhaustiva

    26 Citas (Scopus)

    Resumen

    Introduction: Cutaneous leishmaniasis (CL) is a tropical disease that affects millions of individuals worldwide. The current drugs for CL may be effective but have serious side effects; hence, alternatives are urgently needed. Although plant-derived materials are used for the treatment of various diseases in 80% of the global population, the validation of these products is essential. Gelatin capsules containing dried Artemisia annua L. leaf powder were recently developed as a new herbal formulation (totum) for the oral treatment of malaria and other parasitic diseases. Here, we aimed to determine the usefulness of A. annua gel capsules in CL. Methods: The antileishmanial activity and cytotoxicity of A. annua L. capsules was determined via in vitro and in vivo studies. Moreover, a preliminary evaluation of its therapeutic potential as antileishmanial treatment in humans was conducted in 2 patients with uncomplicated CL. Results: Artemisia annua L. capsules showed moderate in vitro activity in amastigotes of Leishmania (Viannia) panamensis; no cytotoxicity in U-937 macrophages or genotoxicity in human lymphocytes was observed. Five of 6 (83.3%) hamsters treated with A. annua capsules (500mg/kg/day) for 30 days were cured, and the 2 examined patients were cured 45 days after initiation of treatment with 30g of A. annua L. capsules, without any adverse reactions. Both patients remained disease-free 26 and 24 months after treatment completion. Conclusion: Capsules of A. annua L. represent an effective treatment for uncomplicated CL, although further randomized controlled trials are needed to validate its efficacy and safety.

    Idioma originalInglés
    Páginas (desde-hasta)52-60
    Número de páginas9
    PublicaciónRevista da Sociedade Brasileira de Medicina Tropical
    Volumen50
    N.º1
    DOI
    EstadoPublicada - 2017

    Nota bibliográfica

    Publisher Copyright:
    © 2017, Sociedade Brasileira de Medicina Tropical. All rights reserved.

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