TY - JOUR
T1 - Insect growth regulatory activity of Blechnum chilense
AU - Hincapié L, Carlos A.
AU - Monsalve F, Zulma
AU - Parada, Katherine
AU - Lamilla, Claudio
AU - Alarcón, Julio
AU - Céspedes A, Carlos L.
AU - Seigler, David
PY - 2011
Y1 - 2011
N2 - The genus Blechnum has 13 species that are common plants, well-distributed in Chile. Here, we report a phytochemical analysis of B. chilense (Kaulf.) Mett., as well as the insecticidal effects of extracts of this plant. From the n-hexane fraction four phytoecdysones were isolated: ecdysone, ponasterone, shidasterone and 2-deoxycrustecdysone. A bioassay with Drosophila melanogaster larvae was used to evaluate insecticidal activity. The EtOAc and n-hexane fractions at 800 ppm caused 66.7 and 50.0% larval mortality, respectively. Treatments with both extracts at 800 ppm caused the greatest larval mortality, whereas treatments with 500 and 200 ppm induced premature pupation compared with the control and the highest adult mortality, probably due to interference with ecdysteroid metabolism and inhibition of ecdysis triggering hormone (ETH). The dead adult flies exhibited malformations.
AB - The genus Blechnum has 13 species that are common plants, well-distributed in Chile. Here, we report a phytochemical analysis of B. chilense (Kaulf.) Mett., as well as the insecticidal effects of extracts of this plant. From the n-hexane fraction four phytoecdysones were isolated: ecdysone, ponasterone, shidasterone and 2-deoxycrustecdysone. A bioassay with Drosophila melanogaster larvae was used to evaluate insecticidal activity. The EtOAc and n-hexane fractions at 800 ppm caused 66.7 and 50.0% larval mortality, respectively. Treatments with both extracts at 800 ppm caused the greatest larval mortality, whereas treatments with 500 and 200 ppm induced premature pupation compared with the control and the highest adult mortality, probably due to interference with ecdysteroid metabolism and inhibition of ecdysis triggering hormone (ETH). The dead adult flies exhibited malformations.
KW - Blechnum chilense
KW - Insecticidal activity
KW - Phytoecdysteroids
UR - http://www.scopus.com/inward/record.url?scp=80054789847&partnerID=8YFLogxK
M3 - Artículo en revista científica indexada
C2 - 21922904
AN - SCOPUS:80054789847
SN - 1934-578X
VL - 6
SP - 1085
EP - 1088
JO - Natural Product Communications
JF - Natural Product Communications
IS - 8
ER -