Embedding graphic content in multimedia through steganography is a useful and fast practice to hide information. However, detecting the use of this technique is complex and sometimes unsuccessful because variations are not visually perceptible. This article proposes the use of a binary classification model based on artificial neural networks to detect the presence of LSB steganography on monochromatic still images of 256x256 and 8 bits, based on the Standford Genome Project. The steganograms were generated by varying the payload from 0.1 to 0.5 to obtain image pairs of carriers and steganograms. For each steganogram, the following features were extracted from image histograms: kurtosis, skewness, standard deviation, range, median, harmonic mean, Hjorth mobility, and complexity. The results show that the classifier reaches a 91.45% accuracy in detecting LSB steganography when learning from all payloads, as well as a 96.78% individual classification accuracy in the best case with a payload of 0.5.
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