Molecular identification of shiitake [Lentinula edodes Berk (Pegler)] and production of secondary metabolites with biotechnological potential

Byron Duran-Rivera, Felipe Rojas-Rodas, Wilber Silva-Lopez, Crhistian Gomez-Suarez, Dagoberto Castro-Restrepo

    Producción científica: Contribución a una revistaArtículo en revista científica indexadarevisión exhaustiva

    1 Cita (Scopus)


    The shitake mushroom (Lentinula edodes) is the second most-consumed mushroom in the world; in Colombia, it is cultivated and commercialized on a small scale in some supermarkets. Little is known about the procedence, nutritional and medicinal properties of Shiitake produced in Colombia. In this study, four shiitake isolates grown in Colombia (LEUCO1, LEUCO2, LEUCO3, and LEUCO4) were sequenced in their ITS genes and evaluated for the production of three medicinal metabolites, eritadenine, ergotioneine and β-glucans (1,3-1, 6), using submerged culture. Genetic analysis revealed that all the isolates were close and related to the Japanese strain Cr62. LEUCO1 and LEUCO2 showed a distance of 0.000, as well as LEUCO3 and LEUCO4. All four isolates produced erythadenine in a range of 26.3-8.6 mg / L, with the best performance of LEUCO1 at 26.3 mg / L (p <0.05). Ergotioneine was produced with similar statistical yields in all the isolates with an average of 0.7 mg / g of dry weight biomass (DW). B-glucans (1.3-1.6) were produced with yields of 5.6 - 3.8% of DW biomass, with the best values for LEUCO2 and the lowest for LEUCO4 (p <0.05). In conclusion, we identified low genetic diversity in the four isolates, corresponding to two haplotypes with minimal genetic difference between them, related to the Japanese strain Cr62, indicating that Colombian farmers cultivate almost the same strains of shitake. Secondary metabolites, eritadenine, β-glucans and ergotioneine were found in promising yields useful for the pharmaceutical and food industries. More studies should be conducted to improve the yield of shitake metabolites through new growing conditions for industrial production and to find metabolic pathways and related genes.

    Idioma originalInglés
    Páginas (desde-hasta)1183-1188
    Número de páginas6
    EstadoPublicada - 2020

    Nota bibliográfica

    Publisher Copyright:
    © 2020 Revista Bionatura. All rights reserved.

    Tipos de Productos Minciencias

    • Artículos de investigación con calidad A1 / Q1


    Profundice en los temas de investigación de 'Molecular identification of shiitake [Lentinula edodes Berk (Pegler)] and production of secondary metabolites with biotechnological potential'. En conjunto forman una huella única.

    Citar esto