Optimización de un procedimiento de extracción de ADN para mosquitos anofelinos

Doris A. Rosero; Lina A. Gutiérrez; Astrid V. Cienfuegos; Luz M. Jaramillo; Margarita M. Correa

Optimización de un procedimiento de extracción de ADN para mosquitos anofelinos

Doris A. Rosero, Lina A. Gutiérrez, Astrid V. Cienfuegos, Luz M. Jaramillo, Margarita M. Correa

Resultado de la investigación: Contribución a una revista › Artículo › revisión exhaustiva

24 Citas (Scopus)

Resumen

A DNA extraction protocol for anopheline mosquitoes was optimized by the evaluation of various incubation times of the sample with Potassium acetate (KAc). The results showed that the highest DNA concentration and amplification frequency were obtained with the 1 h treatment, which suggests that is possible to reduce the incubation time with KAc without affecting the amount and quality of the DNA extracted.

Título traducido de la contribución	Optimization of a DNA extraction procedure for anopheline mosquitoes
Idioma original	Español
Páginas (desde-hasta)	260-263
Número de páginas	4
Publicación	Revista Colombiana de Entomologia
Volumen	36
N.º	2
Estado	Publicada - jul. 2010
Publicado de forma externa	Sí

Palabras clave

Anopheles
PCR
Potassium Acetate

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abstract = "A DNA extraction protocol for anopheline mosquitoes was optimized by the evaluation of various incubation times of the sample with Potassium acetate (KAc). The results showed that the highest DNA concentration and amplification frequency were obtained with the 1 h treatment, which suggests that is possible to reduce the incubation time with KAc without affecting the amount and quality of the DNA extracted.",

keywords = "Anopheles, PCR, Potassium Acetate",

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T1 - Optimización de un procedimiento de extracción de ADN para mosquitos anofelinos

AU - Rosero, Doris A.

AU - Gutiérrez, Lina A.

AU - Cienfuegos, Astrid V.

AU - Jaramillo, Luz M.

AU - Correa, Margarita M.

PY - 2010/7

Y1 - 2010/7

N2 - A DNA extraction protocol for anopheline mosquitoes was optimized by the evaluation of various incubation times of the sample with Potassium acetate (KAc). The results showed that the highest DNA concentration and amplification frequency were obtained with the 1 h treatment, which suggests that is possible to reduce the incubation time with KAc without affecting the amount and quality of the DNA extracted.

AB - A DNA extraction protocol for anopheline mosquitoes was optimized by the evaluation of various incubation times of the sample with Potassium acetate (KAc). The results showed that the highest DNA concentration and amplification frequency were obtained with the 1 h treatment, which suggests that is possible to reduce the incubation time with KAc without affecting the amount and quality of the DNA extracted.

KW - Anopheles

KW - PCR

KW - Potassium Acetate

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M3 - Artículo

AN - SCOPUS:79960482794

VL - 36

SP - 260

EP - 263

JO - Revista Colombiana de Entomologia

JF - Revista Colombiana de Entomologia

SN - 0120-0488

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ER -

Optimización de un procedimiento de extracción de ADN para mosquitos anofelinos

Resumen

Palabras clave

Huella

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