A DNA extraction protocol for anopheline mosquitoes was optimized by the evaluation of various incubation times of the sample with Potassium acetate (KAc). The results showed that the highest DNA concentration and amplification frequency were obtained with the 1 h treatment, which suggests that is possible to reduce the incubation time with KAc without affecting the amount and quality of the DNA extracted.
|Título traducido de la contribución||Optimization of a DNA extraction procedure for anopheline mosquitoes|
|Número de páginas||4|
|Publicación||Revista Colombiana de Entomologia|
|Estado||Publicada - jul. 2010|
|Publicado de forma externa||Sí|
- Potassium Acetate