Optimization of a DNA extraction procedure for anopheline mosquitoes

Doris A. Rosero; Lina A. Gutiérrez; Astrid V. Cienfuegos; Luz M. Jaramillo; Margarita M. Correa

Optimization of a DNA extraction procedure for anopheline mosquitoes

Título traducido de la contribución: Optimización de un procedimiento de extracción de ADN para mosquitos anofelinos

Doris A. Rosero, Lina A. Gutiérrez, Astrid V. Cienfuegos, Luz M. Jaramillo, Margarita M. Correa

Producción científica: Contribución a una revista › Artículo en revista científica indexada › revisión exhaustiva

29 Citas (Scopus)

Resumen

A DNA extraction protocol for anopheline mosquitoes was optimized by the evaluation of various incubation times of the sample with Potassium acetate (KAc). The results showed that the highest DNA concentration and amplification frequency were obtained with the 1 h treatment, which suggests that is possible to reduce the incubation time with KAc without affecting the amount and quality of the DNA extracted.

Título traducido de la contribución	Optimización de un procedimiento de extracción de ADN para mosquitos anofelinos
Idioma original	Inglés
Páginas (desde-hasta)	260-263
Número de páginas	4
Publicación	Revista Colombiana de Entomologia
Volumen	36
N.º	2
Estado	Publicada - jul. 2010
Publicado de forma externa	Sí

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title = "Optimization of a DNA extraction procedure for anopheline mosquitoes",

abstract = "A DNA extraction protocol for anopheline mosquitoes was optimized by the evaluation of various incubation times of the sample with Potassium acetate (KAc). The results showed that the highest DNA concentration and amplification frequency were obtained with the 1 h treatment, which suggests that is possible to reduce the incubation time with KAc without affecting the amount and quality of the DNA extracted.",

keywords = "Anopheles, PCR, Potassium Acetate",

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T1 - Optimization of a DNA extraction procedure for anopheline mosquitoes

AU - Rosero, Doris A.

AU - Gutiérrez, Lina A.

AU - Cienfuegos, Astrid V.

AU - Jaramillo, Luz M.

AU - Correa, Margarita M.

PY - 2010/7

Y1 - 2010/7

N2 - A DNA extraction protocol for anopheline mosquitoes was optimized by the evaluation of various incubation times of the sample with Potassium acetate (KAc). The results showed that the highest DNA concentration and amplification frequency were obtained with the 1 h treatment, which suggests that is possible to reduce the incubation time with KAc without affecting the amount and quality of the DNA extracted.

AB - A DNA extraction protocol for anopheline mosquitoes was optimized by the evaluation of various incubation times of the sample with Potassium acetate (KAc). The results showed that the highest DNA concentration and amplification frequency were obtained with the 1 h treatment, which suggests that is possible to reduce the incubation time with KAc without affecting the amount and quality of the DNA extracted.

KW - Anopheles

KW - PCR

KW - Potassium Acetate

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M3 - Artículo en revista científica indexada

AN - SCOPUS:79960482794

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EP - 263

JO - Revista Colombiana de Entomologia

JF - Revista Colombiana de Entomologia

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Optimization of a DNA extraction procedure for anopheline mosquitoes

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