Population structure among Mycobacterium tuberculosis isolates from pulmonary tuberculosis patients in Colombia

Teresa Realpe; Nidia Correa; Juan Carlos Rozo; Beatriz Elena Ferro; Verónica Gomez; Elsa Zapata; Wellman Ribon; Gloria Puerto; Claudia Castro; Luisa María Nieto; Maria Lilia Diaz; Oriana Rivera; David Couvin; Nalin Rastogi; Maria Patricia Arbelaez; Jaime Robledo

doi:10.1371/journal.pone.0093848

Population structure among Mycobacterium tuberculosis isolates from pulmonary tuberculosis patients in Colombia

Teresa Realpe, Nidia Correa, Juan Carlos Rozo, Beatriz Elena Ferro, Verónica Gomez, Elsa Zapata, Wellman Ribon, Gloria Puerto, Claudia Castro, Luisa María Nieto, Maria Lilia Diaz, Oriana Rivera, David Couvin, Nalin Rastogi, Maria Patricia Arbelaez, Jaime Robledo

Unidad de Bacteriología y Microbacterias, CIB-UPB

Producción científica: Contribución a una revista › Artículo en revista científica indexada › revisión exhaustiva

45 Citas (Scopus)

Resumen

Background: Phylogeographic composition of M. tuberculosis populations reveals associations between lineages and human populations that might have implications for the development of strategies to control the disease. In Latin America, lineage 4 or the Euro-American, is predominant with considerable variations among and within countries. In Colombia, although few studies from specific localities have revealed differences in M. tuberculosis populations, there are still areas of the country where this information is lacking, as is a comparison of Colombian isolates with those from the rest of the world.

Principal Findings: A total of 414 M. tuberculosis isolates from adult pulmonary tuberculosis cases from three Colombian states were studied. Isolates were genotyped using IS6110-restriction fragment length polymorphism (RFLP), spoligotyping, and 24-locus Mycobacterial interspersed repetitive units variable number tandem repeats (MIRU-VNTRs). SIT42 (LAM9) and SIT62 (H1) represented 53.3% of isolates, followed by 8.21% SIT50 (H3), 5.07% SIT53 (T1), and 3.14% SIT727 (H1). Composite spoligotyping and 24-locus MIRU- VNTR minimum spanning tree analysis suggest a recent expansion of SIT42 and SIT62 evolved originally from SIT53 (T1). The proportion of Haarlem sublineage (44.3%) was significantly higher than that in neighboring countries. Associations were found between M. tuberculosis MDR and SIT45 (H1), as well as HIV-positive serology with SIT727 (H1) and SIT53 (T1).

Conclusions: This study showed the population structure of M. tuberculosis in several regions from Colombia with a dominance of the LAM and Haarlem sublineages, particularly in two major urban settings (Medellín and Cali). Dominant spoligotypes were LAM9 (SIT 42) and Haarlem (SIT62). The proportion of the Haarlem sublineage was higher in Colombia compared to that in neighboring countries, suggesting particular conditions of co-evolution with the corresponding human population that favor the success of this sublineage.

Idioma original	Inglés
Número de artículo	e93848
Publicación	PLoS ONE
Volumen	9
N.º	4
DOI	https://doi.org/10.1371/journal.pone.0093848
Estado	Publicada - 18 abr. 2014

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Realpe, T., Correa, N., Rozo, J. C., Ferro, B. E., Gomez, V., Zapata, E., Ribon, W., Puerto, G., Castro, C., Nieto, L. M., Diaz, M. L., Rivera, O., Couvin, D., Rastogi, N., Arbelaez, M. P., & Robledo, J. (2014). Population structure among Mycobacterium tuberculosis isolates from pulmonary tuberculosis patients in Colombia. PLoS ONE, 9(4), Artículo e93848. https://doi.org/10.1371/journal.pone.0093848

@article{9c46512cb0944359916e3f2dd800f9f5,

title = "Population structure among Mycobacterium tuberculosis isolates from pulmonary tuberculosis patients in Colombia",

abstract = "Background: Phylogeographic composition of M. tuberculosis populations reveals associations between lineages and human populations that might have implications for the development of strategies to control the disease. In Latin America, lineage 4 or the Euro-American, is predominant with considerable variations among and within countries. In Colombia, although few studies from specific localities have revealed differences in M. tuberculosis populations, there are still areas of the country where this information is lacking, as is a comparison of Colombian isolates with those from the rest of the world.Principal Findings: A total of 414 M. tuberculosis isolates from adult pulmonary tuberculosis cases from three Colombian states were studied. Isolates were genotyped using IS6110-restriction fragment length polymorphism (RFLP), spoligotyping, and 24-locus Mycobacterial interspersed repetitive units variable number tandem repeats (MIRU-VNTRs). SIT42 (LAM9) and SIT62 (H1) represented 53.3% of isolates, followed by 8.21% SIT50 (H3), 5.07% SIT53 (T1), and 3.14% SIT727 (H1). Composite spoligotyping and 24-locus MIRU- VNTR minimum spanning tree analysis suggest a recent expansion of SIT42 and SIT62 evolved originally from SIT53 (T1). The proportion of Haarlem sublineage (44.3%) was significantly higher than that in neighboring countries. Associations were found between M. tuberculosis MDR and SIT45 (H1), as well as HIV-positive serology with SIT727 (H1) and SIT53 (T1).Conclusions: This study showed the population structure of M. tuberculosis in several regions from Colombia with a dominance of the LAM and Haarlem sublineages, particularly in two major urban settings (Medell{\'i}n and Cali). Dominant spoligotypes were LAM9 (SIT 42) and Haarlem (SIT62). The proportion of the Haarlem sublineage was higher in Colombia compared to that in neighboring countries, suggesting particular conditions of co-evolution with the corresponding human population that favor the success of this sublineage.",

author = "Teresa Realpe and Nidia Correa and Rozo, {Juan Carlos} and Ferro, {Beatriz Elena} and Ver{\'o}nica Gomez and Elsa Zapata and Wellman Ribon and Gloria Puerto and Claudia Castro and Nieto, {Luisa Mar{\'i}a} and Diaz, {Maria Lilia} and Oriana Rivera and David Couvin and Nalin Rastogi and Arbelaez, {Maria Patricia} and Jaime Robledo",

year = "2014",

month = apr,

day = "18",

doi = "10.1371/journal.pone.0093848",

language = "Ingl{\'e}s",

volume = "9",

journal = "PLoS ONE",

issn = "1932-6203",

publisher = "Public Library of Science",

number = "4",

}

Realpe, T, Correa, N, Rozo, JC, Ferro, BE, Gomez, V, Zapata, E, Ribon, W, Puerto, G, Castro, C, Nieto, LM, Diaz, ML, Rivera, O, Couvin, D, Rastogi, N, Arbelaez, MP & Robledo, J 2014, 'Population structure among Mycobacterium tuberculosis isolates from pulmonary tuberculosis patients in Colombia', PLoS ONE, vol. 9, n.º 4, e93848. https://doi.org/10.1371/journal.pone.0093848

TY - JOUR

T1 - Population structure among Mycobacterium tuberculosis isolates from pulmonary tuberculosis patients in Colombia

AU - Realpe, Teresa

AU - Correa, Nidia

AU - Rozo, Juan Carlos

AU - Ferro, Beatriz Elena

AU - Gomez, Verónica

AU - Zapata, Elsa

AU - Ribon, Wellman

AU - Puerto, Gloria

AU - Castro, Claudia

AU - Nieto, Luisa María

AU - Diaz, Maria Lilia

AU - Rivera, Oriana

AU - Couvin, David

AU - Rastogi, Nalin

AU - Arbelaez, Maria Patricia

AU - Robledo, Jaime

PY - 2014/4/18

Y1 - 2014/4/18

N2 - Background: Phylogeographic composition of M. tuberculosis populations reveals associations between lineages and human populations that might have implications for the development of strategies to control the disease. In Latin America, lineage 4 or the Euro-American, is predominant with considerable variations among and within countries. In Colombia, although few studies from specific localities have revealed differences in M. tuberculosis populations, there are still areas of the country where this information is lacking, as is a comparison of Colombian isolates with those from the rest of the world.Principal Findings: A total of 414 M. tuberculosis isolates from adult pulmonary tuberculosis cases from three Colombian states were studied. Isolates were genotyped using IS6110-restriction fragment length polymorphism (RFLP), spoligotyping, and 24-locus Mycobacterial interspersed repetitive units variable number tandem repeats (MIRU-VNTRs). SIT42 (LAM9) and SIT62 (H1) represented 53.3% of isolates, followed by 8.21% SIT50 (H3), 5.07% SIT53 (T1), and 3.14% SIT727 (H1). Composite spoligotyping and 24-locus MIRU- VNTR minimum spanning tree analysis suggest a recent expansion of SIT42 and SIT62 evolved originally from SIT53 (T1). The proportion of Haarlem sublineage (44.3%) was significantly higher than that in neighboring countries. Associations were found between M. tuberculosis MDR and SIT45 (H1), as well as HIV-positive serology with SIT727 (H1) and SIT53 (T1).Conclusions: This study showed the population structure of M. tuberculosis in several regions from Colombia with a dominance of the LAM and Haarlem sublineages, particularly in two major urban settings (Medellín and Cali). Dominant spoligotypes were LAM9 (SIT 42) and Haarlem (SIT62). The proportion of the Haarlem sublineage was higher in Colombia compared to that in neighboring countries, suggesting particular conditions of co-evolution with the corresponding human population that favor the success of this sublineage.

AB - Background: Phylogeographic composition of M. tuberculosis populations reveals associations between lineages and human populations that might have implications for the development of strategies to control the disease. In Latin America, lineage 4 or the Euro-American, is predominant with considerable variations among and within countries. In Colombia, although few studies from specific localities have revealed differences in M. tuberculosis populations, there are still areas of the country where this information is lacking, as is a comparison of Colombian isolates with those from the rest of the world.Principal Findings: A total of 414 M. tuberculosis isolates from adult pulmonary tuberculosis cases from three Colombian states were studied. Isolates were genotyped using IS6110-restriction fragment length polymorphism (RFLP), spoligotyping, and 24-locus Mycobacterial interspersed repetitive units variable number tandem repeats (MIRU-VNTRs). SIT42 (LAM9) and SIT62 (H1) represented 53.3% of isolates, followed by 8.21% SIT50 (H3), 5.07% SIT53 (T1), and 3.14% SIT727 (H1). Composite spoligotyping and 24-locus MIRU- VNTR minimum spanning tree analysis suggest a recent expansion of SIT42 and SIT62 evolved originally from SIT53 (T1). The proportion of Haarlem sublineage (44.3%) was significantly higher than that in neighboring countries. Associations were found between M. tuberculosis MDR and SIT45 (H1), as well as HIV-positive serology with SIT727 (H1) and SIT53 (T1).Conclusions: This study showed the population structure of M. tuberculosis in several regions from Colombia with a dominance of the LAM and Haarlem sublineages, particularly in two major urban settings (Medellín and Cali). Dominant spoligotypes were LAM9 (SIT 42) and Haarlem (SIT62). The proportion of the Haarlem sublineage was higher in Colombia compared to that in neighboring countries, suggesting particular conditions of co-evolution with the corresponding human population that favor the success of this sublineage.

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U2 - 10.1371/journal.pone.0093848

DO - 10.1371/journal.pone.0093848

M3 - Artículo en revista científica indexada

C2 - 24747767

AN - SCOPUS:84899684731

SN - 1932-6203

VL - 9

JO - PLoS ONE

JF - PLoS ONE

IS - 4

M1 - e93848

ER -

Population structure among Mycobacterium tuberculosis isolates from pulmonary tuberculosis patients in Colombia

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