Protocol for the selection of Mycobacterium tuberculosis spontaneous resistant mutants to D-cycloserine

Alejandra Osorio-González, Nataly Álvarez, Teresa De Jesus Realpe Quintero, Jaime Robledo

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Resumen

Mycobacterium tuberculosis (MTB) is known for its adaptive capability in developing resistance to antibiotics, through the selection of spontaneous mutations that arise during treatment. Generating spontaneous antibiotic-resistant mutants in vitro is challenging but necessary for studying this phenomenon. A protocol was designed and tested to select stable, MTB spontaneous, d-cycloserine (DCS) resistant mutants. Twenty-four colonies resistant to DCS were selected, demonstrating an increase between 1 and 4 times the Minimum Inhibitory Concentration (MIC) set for Mycobacterium tuberculosis H37Rv ATCC 27294 reference strain.
Idioma originalInglés
Número de artículo102690
PublicaciónMethodsX
Volumen12
DOI
EstadoPublicada - jun. 2024

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