Protocol for the selection of Mycobacterium tuberculosis spontaneous resistant mutants to D-cycloserine

Alejandra Osorio-González; Nataly Álvarez; Teresa Realpe; Jaime Robledo

doi:10.1016/j.mex.2024.102690

Protocol for the selection of Mycobacterium tuberculosis spontaneous resistant mutants to D-cycloserine

Alejandra Osorio-González
, Nataly Álvarez
, Teresa Realpe
, Jaime Robledo

Unidad de Bacteriología y Microbacterias, CIB-UPB

Producción científica: Contribución a una revista científica › Artículo en revista científica indexada › revisión exhaustiva

Resumen

Mycobacterium tuberculosis (MTB) is known for its adaptive capability in developing resistance to antibiotics, through the selection of spontaneous mutations that arise during treatment. Generating spontaneous antibiotic-resistant mutants in vitro is challenging but necessary for studying this phenomenon. A protocol was designed and tested to select stable, MTB spontaneous, d-cycloserine (DCS) resistant mutants. Twenty-four colonies resistant to DCS were selected, demonstrating an increase between 1 and 4 times the Minimum Inhibitory Concentration (MIC) set for Mycobacterium tuberculosis H37Rv ATCC 27294 reference strain.

Idioma original	Inglés
Número de artículo	102690
Publicación	MethodsX
Volumen	12
DOI	https://doi.org/10.1016/j.mex.2024.102690
Estado	Publicada - jun. 2024

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© 2024

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title = "Protocol for the selection of Mycobacterium tuberculosis spontaneous resistant mutants to D-cycloserine",

abstract = "Mycobacterium tuberculosis (MTB) is known for its adaptive capability in developing resistance to antibiotics, through the selection of spontaneous mutations that arise during treatment. Generating spontaneous antibiotic-resistant mutants in vitro is challenging but necessary for studying this phenomenon. A protocol was designed and tested to select stable, MTB spontaneous, d-cycloserine (DCS) resistant mutants. Twenty-four colonies resistant to DCS were selected, demonstrating an increase between 1 and 4 times the Minimum Inhibitory Concentration (MIC) set for Mycobacterium tuberculosis H37Rv ATCC 27294 reference strain.",

keywords = "Antibiotics, Drug-resistant, H37Rv, Mycobacterium tuberculosis, Spontaneous mutants",

author = "Alejandra Osorio-Gonz{\'a}lez and Nataly {\'A}lvarez and Teresa Realpe and Jaime Robledo",

note = "Publisher Copyright: {\textcopyright} 2024",

year = "2024",

month = jun,

doi = "10.1016/j.mex.2024.102690",

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volume = "12",

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TY - JOUR

T1 - Protocol for the selection of Mycobacterium tuberculosis spontaneous resistant mutants to D-cycloserine

AU - Osorio-González, Alejandra

AU - Álvarez, Nataly

AU - Realpe, Teresa

AU - Robledo, Jaime

PY - 2024/6

Y1 - 2024/6

N2 - Mycobacterium tuberculosis (MTB) is known for its adaptive capability in developing resistance to antibiotics, through the selection of spontaneous mutations that arise during treatment. Generating spontaneous antibiotic-resistant mutants in vitro is challenging but necessary for studying this phenomenon. A protocol was designed and tested to select stable, MTB spontaneous, d-cycloserine (DCS) resistant mutants. Twenty-four colonies resistant to DCS were selected, demonstrating an increase between 1 and 4 times the Minimum Inhibitory Concentration (MIC) set for Mycobacterium tuberculosis H37Rv ATCC 27294 reference strain.

AB - Mycobacterium tuberculosis (MTB) is known for its adaptive capability in developing resistance to antibiotics, through the selection of spontaneous mutations that arise during treatment. Generating spontaneous antibiotic-resistant mutants in vitro is challenging but necessary for studying this phenomenon. A protocol was designed and tested to select stable, MTB spontaneous, d-cycloserine (DCS) resistant mutants. Twenty-four colonies resistant to DCS were selected, demonstrating an increase between 1 and 4 times the Minimum Inhibitory Concentration (MIC) set for Mycobacterium tuberculosis H37Rv ATCC 27294 reference strain.

KW - Antibiotics

KW - Drug-resistant

KW - H37Rv

KW - Mycobacterium tuberculosis

KW - Spontaneous mutants

UR - https://www.scopus.com/pages/publications/85190093964

U2 - 10.1016/j.mex.2024.102690

DO - 10.1016/j.mex.2024.102690

M3 - Artículo en revista científica indexada

AN - SCOPUS:85190093964

SN - 2215-0161

VL - 12

JO - MethodsX

JF - MethodsX

M1 - 102690

ER -

Protocol for the selection of Mycobacterium tuberculosis spontaneous resistant mutants to D-cycloserine

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