Rapid detection of rifampicin and isoniazid resistance in Mycobacterium tuberculosis by the direct thin-layer agar method

Jaime Robledo; G. I. Mejia; L. Paniagua; A. Martin; A. Guzmán

Rapid detection of rifampicin and isoniazid resistance in Mycobacterium tuberculosis by the direct thin-layer agar method

Jaime Robledo, G. I. Mejia, L. Paniagua, A. Martin, A. Guzmán

Producción científica: Contribución a una revista › Artículo en revista científica indexada › revisión exhaustiva

37 Citas (Scopus)

Resumen

We evaluated thin-layer agar (TLA) for the detection of resistance of Mycobacterium tuberculosis to rifampicin (RMP) and isoniazid (INH) as a direct method in patients at risk of multidrug-resistant tuberculosis (MDR-TB). Quadrant TLA plates contain 7H10 Middlebrook growth control, para-nitrobenzoic acid, INH and RMP. Detection of RMP and INH resistance by TLA was compared to that in indirect conventional drug susceptibility testing (DST) and conventional culture media. Median time for growth was respectively 22, 10 and 7.6 days for Löwenstein-Jensen, TLA and the Mycobacterial Growth Indicator Tube. TLA sensitivity, specificity and predictive values for RMP and INH resistance were 100%. Time to resistance detection was respectively 11 and 11.5 days for RMP and INH. TLA showed a rapid turnaround time and performance comparable to conventional DST methods.

Idioma original	Inglés
Páginas (desde-hasta)	1482-1484
Número de páginas	3
Publicación	International Journal of Tuberculosis and Lung Disease
Volumen	12
N.º	12
Estado	Publicada - dic. 2008

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title = "Rapid detection of rifampicin and isoniazid resistance in Mycobacterium tuberculosis by the direct thin-layer agar method",

abstract = "We evaluated thin-layer agar (TLA) for the detection of resistance of Mycobacterium tuberculosis to rifampicin (RMP) and isoniazid (INH) as a direct method in patients at risk of multidrug-resistant tuberculosis (MDR-TB). Quadrant TLA plates contain 7H10 Middlebrook growth control, para-nitrobenzoic acid, INH and RMP. Detection of RMP and INH resistance by TLA was compared to that in indirect conventional drug susceptibility testing (DST) and conventional culture media. Median time for growth was respectively 22, 10 and 7.6 days for L{\"o}wenstein-Jensen, TLA and the Mycobacterial Growth Indicator Tube. TLA sensitivity, specificity and predictive values for RMP and INH resistance were 100%. Time to resistance detection was respectively 11 and 11.5 days for RMP and INH. TLA showed a rapid turnaround time and performance comparable to conventional DST methods.",

keywords = "Drug susceptibility testing, MDR-TB, Rapid DST",

author = "Jaime Robledo and Mejia, {G. I.} and L. Paniagua and A. Martin and A. Guzm{\'a}n",

year = "2008",

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language = "Ingl{\'e}s",

volume = "12",

pages = "1482--1484",

journal = "International Journal of Tuberculosis and Lung Disease",

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Rapid detection of rifampicin and isoniazid resistance in Mycobacterium tuberculosis by the direct thin-layer agar method. / Robledo, Jaime; Mejia, G. I.; Paniagua, L. et al.
En: International Journal of Tuberculosis and Lung Disease, Vol. 12, N.º 12, 12.2008, p. 1482-1484.

Producción científica: Contribución a una revista › Artículo en revista científica indexada › revisión exhaustiva

TY - JOUR

T1 - Rapid detection of rifampicin and isoniazid resistance in Mycobacterium tuberculosis by the direct thin-layer agar method

AU - Robledo, Jaime

AU - Mejia, G. I.

AU - Paniagua, L.

AU - Martin, A.

AU - Guzmán, A.

PY - 2008/12

Y1 - 2008/12

N2 - We evaluated thin-layer agar (TLA) for the detection of resistance of Mycobacterium tuberculosis to rifampicin (RMP) and isoniazid (INH) as a direct method in patients at risk of multidrug-resistant tuberculosis (MDR-TB). Quadrant TLA plates contain 7H10 Middlebrook growth control, para-nitrobenzoic acid, INH and RMP. Detection of RMP and INH resistance by TLA was compared to that in indirect conventional drug susceptibility testing (DST) and conventional culture media. Median time for growth was respectively 22, 10 and 7.6 days for Löwenstein-Jensen, TLA and the Mycobacterial Growth Indicator Tube. TLA sensitivity, specificity and predictive values for RMP and INH resistance were 100%. Time to resistance detection was respectively 11 and 11.5 days for RMP and INH. TLA showed a rapid turnaround time and performance comparable to conventional DST methods.

AB - We evaluated thin-layer agar (TLA) for the detection of resistance of Mycobacterium tuberculosis to rifampicin (RMP) and isoniazid (INH) as a direct method in patients at risk of multidrug-resistant tuberculosis (MDR-TB). Quadrant TLA plates contain 7H10 Middlebrook growth control, para-nitrobenzoic acid, INH and RMP. Detection of RMP and INH resistance by TLA was compared to that in indirect conventional drug susceptibility testing (DST) and conventional culture media. Median time for growth was respectively 22, 10 and 7.6 days for Löwenstein-Jensen, TLA and the Mycobacterial Growth Indicator Tube. TLA sensitivity, specificity and predictive values for RMP and INH resistance were 100%. Time to resistance detection was respectively 11 and 11.5 days for RMP and INH. TLA showed a rapid turnaround time and performance comparable to conventional DST methods.

KW - Drug susceptibility testing

KW - MDR-TB

KW - Rapid DST

UR - http://www.scopus.com/inward/record.url?scp=58049199489&partnerID=8YFLogxK

M3 - Artículo en revista científica indexada

C2 - 19017461

AN - SCOPUS:58049199489

SN - 1027-3719

VL - 12

SP - 1482

EP - 1484

JO - International Journal of Tuberculosis and Lung Disease

JF - International Journal of Tuberculosis and Lung Disease

IS - 12

ER -

Rapid detection of rifampicin and isoniazid resistance in Mycobacterium tuberculosis by the direct thin-layer agar method

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