Revisiting the reference genomes of human pathogenic Cryptosporidium species: Reannotation of C. parvum Iowa and a new C. hominis reference

Juan P. Isaza, Ana Luz Galvan, Victor Polanco, Bernice Huang, Andrey V. Matveyev, Myrna G. Serrano, Patricio Manque, Gregory A. Buck, Juan F. Alzate

Resultado de la investigación: Contribución a una revistaArtículorevisión exhaustiva

33 Citas (Scopus)

Resumen

Cryptosporidium parvum and C. hominis are the most relevant species of this genus for human health. Both cause a self-limiting diarrhea in immunocompetent individuals, but cause potentially life-threatening disease in the immunocompromised. Despite the importance of these pathogens, only one reference genome of each has been analyzed and published. These two reference genomes were sequenced using automated capillary sequencing; as of yet, no next generation sequencing technology has been applied to improve their assemblies and annotations. For C. hominis, the main challenge that prevents a larger number of genomes to be sequenced is its resistance to axenic culture. In the present study, we employed next generation technology to analyse the genomic DNA and RNA to generate a new reference genome sequence of a C. hominis strain isolated directly from human stool and a new genome annotation of the C. parvum Iowa reference genome.

Idioma originalInglés
Número de artículo16324
PublicaciónScientific Reports
Volumen5
DOI
EstadoPublicada - 9 nov. 2015
Publicado de forma externa

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