TY - JOUR
T1 - Sputum induction is a safe procedure to use in prisoners and MGIT is the best culture method to diagnose tuberculosis in prisons
T2 - A cohort study
AU - Rueda, Zulma Vanessa
AU - López, Lucelly
AU - Marín, Diana
AU - Vélez, Lázaro A.
AU - Arbeláez, María Patricia
N1 - Publisher Copyright:
© 2015 The Authors.
PY - 2015/4/1
Y1 - 2015/4/1
N2 - •Induced sputum is a safe procedure in prisons.•The concordance between induced sputum and spontaneous sputum is good.•MGIT had the highest percentage of positive cultures.•MGIT reduced the time to detection of Mycobacterium tuberculosis. Objectives: To evaluate the concordance and safety of induced sputum (IS) and spontaneous sputum (SS), and estimate concordance and time to detection of M. tuberculosis between Lowenstein-Jensen (LJ), thin-layer agar (TLA), and the Mycobacteria Growth Indicator Tube system (MGIT). Methods: This was a cohort study. Prisoners with pulmonary tuberculosis (PTB) were followed for 2 years. At baseline and every follow-up visit, three sputum samples were taken on consecutive days (one IS and two SS) and adverse events occurring before, during, and 30. min after IS were registered. All sputum samples were stained with auramine and cultured in LJ, TLA (to test resistance), and MGIT. Results: Five hundred eighty-six IS and 532 SS were performed on 64 PTB patients. Breathlessness (1.6%), cough (1.2%), hemoptysis (0.3%), and cyanosis (0.2%) were the only complications. Concordance between IS and SS was 0.78 (95% confidence interval 0.69-0.87); 11 positive cultures from IS samples were negative in SS, and 11 positive cultures from SS samples were negative in IS. One hundred seventy-eight cultures were positive by any technique: MGIT 95%, LJ 73%, and TLA 57%. Time to detection of M. tuberculosis in LJ, TLA, and MGIT was 31, 18, and 11 days, respectively. Conclusions: The IS procedure is safe in prisons. The MGIT system is better and faster than LJ and TLA in the diagnosis of M. tuberculosis.
AB - •Induced sputum is a safe procedure in prisons.•The concordance between induced sputum and spontaneous sputum is good.•MGIT had the highest percentage of positive cultures.•MGIT reduced the time to detection of Mycobacterium tuberculosis. Objectives: To evaluate the concordance and safety of induced sputum (IS) and spontaneous sputum (SS), and estimate concordance and time to detection of M. tuberculosis between Lowenstein-Jensen (LJ), thin-layer agar (TLA), and the Mycobacteria Growth Indicator Tube system (MGIT). Methods: This was a cohort study. Prisoners with pulmonary tuberculosis (PTB) were followed for 2 years. At baseline and every follow-up visit, three sputum samples were taken on consecutive days (one IS and two SS) and adverse events occurring before, during, and 30. min after IS were registered. All sputum samples were stained with auramine and cultured in LJ, TLA (to test resistance), and MGIT. Results: Five hundred eighty-six IS and 532 SS were performed on 64 PTB patients. Breathlessness (1.6%), cough (1.2%), hemoptysis (0.3%), and cyanosis (0.2%) were the only complications. Concordance between IS and SS was 0.78 (95% confidence interval 0.69-0.87); 11 positive cultures from IS samples were negative in SS, and 11 positive cultures from SS samples were negative in IS. One hundred seventy-eight cultures were positive by any technique: MGIT 95%, LJ 73%, and TLA 57%. Time to detection of M. tuberculosis in LJ, TLA, and MGIT was 31, 18, and 11 days, respectively. Conclusions: The IS procedure is safe in prisons. The MGIT system is better and faster than LJ and TLA in the diagnosis of M. tuberculosis.
KW - Culture techniques
KW - Diagnosis
KW - Induced sputum
KW - Prisons
KW - Spontaneous sputum
KW - Tuberculosis
UR - http://www.scopus.com/inward/record.url?scp=84922197638&partnerID=8YFLogxK
U2 - 10.1016/j.ijid.2015.01.004
DO - 10.1016/j.ijid.2015.01.004
M3 - Artículo en revista científica indexada
C2 - 25578262
AN - SCOPUS:84922197638
SN - 1201-9712
VL - 33
SP - 82
EP - 88
JO - International Journal of Infectious Diseases
JF - International Journal of Infectious Diseases
ER -
